Reusable biosensors via in situ electrochemical surface regeneration in microfluidic applications
Reusable biosensors via in situ electrochemical surface regeneration in microfluidic applications
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DOI：
10.1016/j.bios.2009.08.003
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发表时间：
2009-10-15
影响因子：
12.6
通讯作者：
Chae, Junseok
中科院分区：
文献类型：
--
作者：
Choi, Seokheun;Chae, Junseok
生物传感器支持生物和临床诊断应用，提供高准确度、精密度和功能性。可重复使用的封闭微流体生物传感器具有挑战性，因为再生方法必须确保回收后的精密度和灵敏度。自组装单分子膜(SAM)和电化学过程显示出很好的应用前景；中链和长链SAM是常用的，但在解吸过程中，再吸附和非特异性吸附会降低生物传感器的性能，限制了可重复使用。克服这些限制是这项工作的重点。我们使用密集堆积的短链自组装膜测试了一种原型和再生方法。与可控的表面粗糙度一起使用，它们减少了再吸附和非特定分子吸附。结果证实，在封闭的微流控装置中形成了短链SAM，解吸促进了表面的清洁循环长达50次。通过表面等离子体共振(SPR)对传感器的重用性进行了现场和实时评估，相对标准偏差(RSD)小于0.82%，表明可重复使用的微流控生物传感器终于实现了。(C)2009爱思唯尔B.V.保留所有权利。
Biosensors support biological and clinical diagnostic applications, offering high accuracy, precision, and functionality. Viable reusable biosensors for enclosed microfluidics are challenging because regeneration methods must ensure precision and sensitivity after recycle. Self-Assembled Monolayers (SAMs) and electrochemical processes show promise; intermediate and long chain SAMs are commonly used, but during desorption, re-adsorption and non-specific adsorption degrades biosensors, limiting reusability. Overcoming these limits is the focus of this work. We tested a prototype and regeneration method using densely packed, short-chain SAMs. Used in conjunction with controlled surface roughness, they reduced re-adsorption and non-specific molecule adsorption. Results confirmed short-chain SAM formation in enclosed microfluidic devices, and desorption promoted "clean" surface recycling for up to 50 cycles. Sensor reusability was evaluated in situ and in real time by Surface Plasmon Resonance (SPR), with a Relative Standard Deviation (RSD) of less than 0.82%, suggesting reusable microfluidics biosensors are finally within reach. (C) 2009 Elsevier B.V. All rights reserved.