DNA chip-based expression profile analysis indicates involvement of the phosphatidylinositol signaling pathway in multiple plant responses to hormone and abiotic treatments
DNA chip-based expression profile analysis indicates involvement of the phosphatidylinositol signaling pathway in multiple plant responses to hormone and abiotic treatments
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DOI：
10.1038/sj.cr.7290200
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发表时间：
2004-02-01
期刊：
影响因子：
44.1
通讯作者：
Xue, HW
中科院分区：
文献类型：
--
作者：
Lin, WH;Ye, R;Xue, HW
磷脂酰肌醇（PI）代谢途径被认为是植物对许多环境因子的反应中的关键，并且先前的研究表明多个PI相关基因家族参与细胞反应。通过对拟南芥基因组的详细分析，鉴定出82个多肽参与PI信号传导。这些可以分为不同的家族，包括PI磷酸酶（PIS）、PI-磷酸激酶（PIPK）、磷脂酶（PL）、肌醇多磷酸磷酸酶（IPP 3）、肌醇多磷酸激酶（IPK）、PI转移蛋白和推定的肌醇多磷酸受体。PIPK、PLC、PLD和IPP 3等10多种亚型的存在表明，这些基因可能在植物细胞反应或生长发育过程中差异表达。因此，DNA芯片技术被用来研究各种异构体的表达模式。总共扩增了79个mRNA克隆并用于DNA芯片生成。使用代表多种组织或细胞反应的样品进行表达谱分析。测试样品包括正常的叶、茎和花组织，以及用各种激素（生长素、细胞分裂素、赤霉素、脱落酸和油菜素类固醇）或环境因素（温度、钙、钠、干旱、水杨酸和茉莉酸）处理的植物的叶。结果表明，许多PI通路相关基因在这些实验条件下差异表达。特别是，在许多情况下，每个家族的不同亚型特异性表达，表明它们参与组织特异性和细胞对环境条件的反应。这项工作为相关PI相关蛋白的功能研究提供了一个起点，并可能有助于阐明PI途径在发育和细胞反应中的作用。
The phosphatidylinositol (PI) metabolic pathway is considered critical in plant responses to many environmental factors, and previous studies have indicated the involvement of multiple PI-related gene families during cellular responses. Through a detailed analysis of the Arabidopsis thaliana genome, 82 polypeptides were identified as being involved in PI signaling. These could be grouped into different families including PI synthases (PIS), PI-phosphate kinases (PIPK), phospholipases (PL), inositol polyphosphate phosphatases (IPPase), inositol polyphosphate kinases (IPK), PI transfer proteins and putative inositol polyphosphate receptors. The presence of more than 10 isoforms of PIPK, PLC, PLD and IPPase suggested that these genes might be differentially expressed during plant cellular responses or growth and development. Accordingly, DNA chip technology was employed to study the expression patterns of various isoforms. In total, 79 mRNA clones were amplified and used for DNA chip generation. Expression profile analysis was performed using samples that represented multiple tissues or cellular responses. Tested samples included normal leaf, stem and flower tissues, and leaves from plants treated with various hormones (auxin, cytokinin, gibberellin, abscisic acid and brassinosteroid) or environmental factors (temperature, calcium, sodium, drought, salicylic acid and jasmonic acid). Results showed that many PI pathway-related genes were differentially expressed under these experimental conditions. In particular, the different isoforms of each family were specifically expressed in many cases, suggesting their involvement in tissue specificity and cellular responses to environmental conditions. This work provides a starting point for functional studies of the relevant PI-related proteins and may help shed light onto the role of PI pathways in development and cellular responses.