Quantitative and Noninvasive Assessment of Prenatal X-Ray-Induced CNS Abnormalities Using Magnetic Resonance Imaging
Quantitative and Noninvasive Assessment of Prenatal X-Ray-Induced CNS Abnormalities Using Magnetic Resonance Imaging
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DOI：
10.1667/rr2134.1
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发表时间：
2011-01-01
影响因子：
3.4
通讯作者：
Suhara, Tetsuya
中科院分区：
文献类型：
--
作者：
Saito, Shigeyoshi;Aoki, Ichio;Suhara, Tetsuya
我们的目的是用定量MRI无创性地评估产前X射线诱发的中枢神经系统异常的微血管形成、血脑屏障(BBB)和血脑脊液屏障的形成。将8只孕期雌性SD大鼠随机分为对照组和X射线照射组。新生雄性大鼠20只，出生后随机分为4组，每组5只。为了评估血脑屏障的发育，定量比较了Gd-DTPA对正常和产前照射后1~2周形成期动物T-1的影响。为了评估微血管的异常，我们还使用了定量灌注MRI和MR血管成像。组织学检测BBB(白蛋白)和血管内皮细胞(层粘连蛋白)。产前照射组大鼠脑血流量减少，血管构筑异常。然而，Gd增强MRI和白蛋白染色未观察到血脑屏障和血脑脊液屏障的异常。定量灌注MRI、MR血管成像和Gd增强T-1图可用于评估产前照射后的中枢神经系统功能障碍。这些技术为评估意外或不可避免的产前辐射后患者生命早期阶段的状况提供了重要的诊断信息。(C)2011年，由辐射研究学会提供
Our purpose was to noninvasively assess formation of the microvasculature, blood-brain barrier (BBB) and blood-CSF barrier formation of prenatal X-ray-induced CNS abnormalities using quantitative MRI. Eight pregnant female Sprague-Dawley rats were divided into two groups consisting of control and X-irradiated animals. After birth, 20 neonatal male rats were divided into four groups of five rats. To evaluate the development of the BBB, changes in T-1 induced by Gd-DTPA were compared quantitatively in normal and prenatally irradiated animals in the formative period 1 to 2 weeks after birth. To assess the abnormalities of the microvasculature, quantitative perfusion MRI and MR angiography were also used. Histology was also performed to evaluate the BBB (albumin) and vascular endothelial cells (laminin). Decreased cerebral blood flow (CBF) and angioarchitectonic abnormalities were observed in the prenatally irradiated rats. However, abnormalities of the BBB and blood-CSF barrier were not observed using Gd-enhanced MRI and albumin staining. Quantitative perfusion M RI, MR angiography and Gd-enhanced T-1 mapping are useful for assessing CNS disturbance after prenatal exposure to radiation. These techniques provide important diagnostic information for assessing the condition of patients during the early stages of life after accidental or unavoidable prenatal exposure to radiation. (C) 2011 by Radiation Research Society