Mapping of the novel powdery mildew resistance gene Pm2Mb from Aegilops biuncialis based on ph1b-induced homoeologous recombination
Mapping of the novel powdery mildew resistance gene Pm2Mb from Aegilops biuncialis based on ph1b-induced homoeologous recombination
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DOI：
10.1007/s00122-022-04162-4
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发表时间：
2022-07-13
影响因子：
5.4
通讯作者：
Liu, Wenxuan
中科院分区：
文献类型：
--
作者：
Men, Wenqiang;Fan, Ziwei;Liu, Wenxuan
将一个新的抗白粉病基因Pm2 M B导入普通小麦中，通过对2 M（B）重组体的分子细胞遗传学分析，将其定位于染色体2 M（B）L bin FL 0.49-0.66。双钩山羊草是普通小麦的一种野生近缘种，对白粉病高度抗性。以前的研究表明中国春（CS）-Ae. bicuncialis 2 M（B）二体附加系TA 7733对白粉病具有高抗性，该抗病基因暂命名为Pm2 M B。在这项研究中，共65 CS-Ae。通过ph 1b诱导的同源重组，开发了biuncialis 2 M（B）重组体，并基于2 M（B）特异性的不同标记的存在将它们分成12种不同类型。使用原位杂交和分子标记分析进一步表征每个2 M（B）重组类型的片段大小和断点。通过使用分离物E05接种每个2 M（B）重组衍生的F-2后代来评估每种类型的白粉病应答。结合原位杂交、分子标记和2 M（B）重组体的白粉病抗性数据分析，将Pm2 M B基因定位在2 M（B）长臂的FL 0.49-0.66区间，其中有19个2 M（B）特异标记。在65个2 M（B）重组子中，T-11（T2DS.2DL-2 M（B）L）和T-12（Ti2DS.2DL-2 M（B）L-2DL）含有一个小的2 M（B）L片段，该片段含有Pm2 M B。此外，利用70个2 M（B）特异性标记构建了2 M（B）染色体物理图谱，图谱显示山羊草2 M（B）染色体位于亚中部着丝点。biuncialis通过末端染色体内易位重排。新获得的抗白粉病2 M（B）重组体、2 M（B）特异性分子标记和2 M（B）染色体物理图谱的建立，将有助于小麦抗病育种和Pm2 M B的精细定位与克隆。
Key message A novel powdery mildew resistance gene Pm2Mb from Aegilops biuncialis was transferred into common wheat and mapped to chromosome 2M(b)L bin FL 0.49-0.66 by molecular cytogenetic analysis of 2M(b) recombinants. Aegilops biuncialis, a wild relative of common wheat, is highly resistant to powdery mildew. Previous studies identified that chromosome 2M(b) in Chinese Spring (CS)-Ae. biuncialis 2M(b) disomic addition line TA7733 conferred high resistance to powdery mildew, and the resistance gene was temporarily designated as Pm2Mb. In this study, a total of 65 CS-Ae. biuncialis 2M(b) recombinants were developed by ph1b-induced homoeologous recombination and they were grouped into 12 different types based on the presence of different markers of 2M(b)-specificity. Segment sizes and breakpoints of each 2M(b) recombinant type were further characterized using in situ hybridization and molecular marker analyses. Powdery mildew responses of each type were assessed by inoculation of each 2M(b) recombinant-derived F-2 progenies using the isolate E05. Combined analyses of in situ hybridization, molecular markers and powdery mildew resistance data of the 2M(b) recombinants, the gene Pm2Mb was cytologically located to an interval of FL 0.49-0.66 in the long arm of 2M(b), where 19 2M(b)-specific markers were located. Among the 65 2M(b) recombinants, T-11 (T2DS.2DL-2M(b)L) and T-12 (Ti2DS.2DL-2M(b)L-2DL) contained a small 2M(b)L segment harboring Pm2Mb. Besides, a physical map of chromosome 2M(b) was constructed with 70 2M(b)-specific markers in 10 chromosomal bins and the map showed that submetacentric chromosome 2M(b) of Ae. biuncialis was rearranged by a terminal intrachromosomal translocation. The newly developed 2M(b) recombinants with powdery mildew resistance, the 2M(b)-specific molecular markers and the physical map of chromosome 2M(b) will benefit wheat disease breeding as well as fine mapping and cloning of Pm2Mb.