Effect of formulated Metarhizium anisopliae on eggs and eclosing nymphs of Triatoma infestans
Effect of formulated Metarhizium anisopliae on eggs and eclosing nymphs of Triatoma infestans
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DOI：
10.1111/jen.12183
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发表时间：
2015-02
影响因子：
1.9
通讯作者：
J. Rodrigues;L. S. Lobo;É. Fernandes;C. Luz
中科院分区：
文献类型：
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作者：
J. Rodrigues;L. S. Lobo;É. Fernandes;C. Luz
很少有人知道的杀卵效果的真菌攻击锥蝽媒介的昆虫和成年人。用硅藻土和植物油配制的绿僵菌IP 46分生孢子组合制剂对致病锥蝽卵进行了毒力测定。鸡蛋在接近饱和的相对湿度[>98%相对湿度（RH）]下对真菌感染高度敏感，但在75%RH下不敏感，无论所施用的制剂如何。卵的敏感性随着处理前产卵后胚胎形成期的延长而降低。通过施用用DE +油制备的分生孢子并在> 98%RH孵育，最好地抑制了真菌的最终羽化。此外，与不断接触分生孢子的个体相比，在羽化后仅暴露于处理过的表面24小时内，真菌对孢子的影响较小。这些发现有助于更好地了解M.绿僵菌作为针对T.害虫
Little is known about the ovicidal effects of fungi that attack nymphs and adults of triatomine vectors. A combined formulation of Metarhizium anisopliae IP 46 conidia prepared with diatomaceous earth (DE) and vegetable oil was tested against eggs of Triatoma infestans. Eggs were highly susceptible to fungal infection at relative humidity close to saturation [>98% relative humidity (RH)] but not at 75% RH regardless of the formulation applied. Susceptibility of eggs decreased with longer post‐ovipositional embryonation periods before treatments. The eventual eclosion of nymphs was best suppressed by application of conidia prepared with DE + oil and at a >98% RH incubation. Moreover, nymphs were less affected by the fungus when exposed for only a 24‐h period after eclosion to a treated surface than individuals that were in constant contact with the conidia. These findings contribute to a better understanding of the potential of M. anisopliae as an agent against all developmental stages of T. infestans.