Phylogenetic analysis of members of the genus Aeromonas based on gyrB gene sequences
Phylogenetic analysis of members of the genus Aeromonas based on gyrB gene sequences
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DOI：
10.1099/ijs.0.02443-0
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发表时间：
2003-05-01
影响因子：
2.8
通讯作者：
Martínez-Murcia, AJ
中科院分区：
文献类型：
--
作者：
Yáñez, MA;Catalán, V;Martínez-Murcia, AJ
利用编码DNA促旋酶B亚基的基因gyrB序列对气单胞菌属所有已知种的系统发育关系进行了研究。测定了53株气单胞菌gyrB基因的核苷酸序列，并对其中的一些新分离株进行了16 SrDNA可变区分析。这些结果支持了对气单胞菌科的认识，与类志贺邻单胞菌和其他肠道细菌不同。该系统发育标记揭示了菌株分组与迄今为止描述的所有气单胞菌属物种的分类组织一致。特别是，gyrB的结果与16 S rDNA分析一致，而且，前者表现出更高的能力，以区分物种。目前的分析是有用的不同DNA-DNA杂交集之间的报道的差异的阐明。此外，由于在种内水平上发现的序列多样性，gyrB被提议作为用于同时鉴定种和菌株的有用靶标。总之，gyrB基因已被证明是一个很好的分子计时器属气单胞菌的系统发育研究。
The phylogenetic relationships of all known species of the genus Aeromonas were investigated by using the sequence of gyrB, a gene that encodes the B-subunit of DNA gyrase. Nucleotide sequences of gyrB were determined from 53 Aeromonas strains, including some new isolates, which were also characterized by analysis of the 16S rDNA variable regions. The results support the recognition of the family Aeromonadaceae, as distinct from Plesiomonas shigelloides and other enteric bacteria. This phylogenetic marker revealed strain groupings that are consistent with the taxonomic organization of all Aeromonas species described to date. In particular, gyrB results agreed with 16S rDNA analysis; moreover, the former showed a higher capacity to differentiate between species. The present analysis was useful for the elucidation of reported discrepancies between different DNA-DNA hybridization sets. Additionally, due to the sequence diversity found at the intraspecies level, gyrB is proposed as a useful target for simultaneous identification of species and strains. In conclusion, the gyrB gene has proved to be an excellent molecular chronometer for phylogenetic studies of the genus Aeromonas.