Lipoic acid prevents hypertension, hyperglycemia, and the increase in heart mitochondrial superoxide production
Lipoic acid prevents hypertension, hyperglycemia, and the increase in heart mitochondrial superoxide production
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DOI：
10.1016/s0895-7061(02)03253-3
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发表时间：
2003-03-01
影响因子：
3.2
通讯作者：
de Champlain, J
中科院分区：
文献类型：
--
作者：
EL Midaoui, A;Elimadi, A;de Champlain, J
背景资料：本研究旨在研究饮食中补充α-硫辛酸是否可以预防心脏线粒体超氧化物生成的增加以及与慢性葡萄糖喂养大鼠高血压和胰岛素抵抗发展相关的晚期糖基化终产物（AGE）形成的增加。方法：在4周内，给予或不给予Sprague道利大鼠10%D-葡萄糖溶液，与正常食物饮食或与补充α-硫辛酸的饮食组合。氧化应激通过使用光泽精化学发光法测量心脏线粒体超氧化物产生来评估。AGE的形成也进行了评估，在血浆和aorator.Results：慢性给药的葡萄糖导致的血压增加29%，增加30%，在胰岛素血症增加286%，增加408%，在胰岛素抵抗指数增加。慢性葡萄糖喂养也导致心肌线粒体超氧阴离子产生增加22%，主动脉AGE含量增加63%。增加血压，主动脉AGE含量和心脏线粒体超氧化物的生产被阻止在大鼠喂养葡萄糖补充硫辛酸。同时用硫辛酸治疗也减弱了葡萄糖喂养大鼠中胰岛素水平的升高以及胰岛素抵抗。这些发现表明，α-硫辛酸补充剂预防高血压和高血糖症的发展，可能是通过其抗氧化特性，如通过预防心脏线粒体超氧阴离子产生的增加和慢性葡萄糖缺乏的主动脉中AGE形成所反映的治疗的老鼠(C)2003年美国高血压杂志有限公司
Background: The present study was designed to investigate whether the effects of dietary supplementation with alpha-lipoic acid could prevent the increase in mitochondrial superoxide production in the heart as well as the enhanced formation of advanced glycation end-products (AGE) that are associated with the development of hypertension and insulin resistance in chronically glucose-fed rats.Methods: Sprague Dawley rats were either given or not given a 10% D-glucose solution to drink during 4 weeks, combined either with a normal chow diet or with a-lipoic acid supplemented diet. The oxidative stress was evaluated by measuring the heart mitochondrial superoxide production using the lucigenin chemiluminescence method. The formation of AGE was also assessed in plasma and aorta.Results: Chronic administration of glucose resulted in a 29% increase in blood pressure, 30% increase in glycemia, 286% increase in insulinemia, and 408% increase in insulin resistance index. Chronic glucose feeding also resulted in a 22% greater mitochondrial superoxide anion production in heart and in an increase of 63% in AGE content in aorta. Increases in blood pressure, aorta AGE content and heart mitochondrial superoxide production were prevented in the rats fed glucose supplemented with lipoic acid. The simultaneous treatment with lipoic acid also attenuated the rise in insulin levels as well as in insulin resistance in the glucose fed rats.Conclusion: These findings demonstrate that a-lipoic acid supplementation prevents development of hypertension and hyperglycemia, presumably through its antioxidative properties, as reflected by prevention of an increase in heart mitochondrial superoxide anion production and in AGE formation in the aorta of chronically glucose treated rats. (C) 2003 American Journal of Hypertension, Ltd.