Redox-Responsive Resilin-Like Hydrogels for Tissue Engineering and Drug Delivery Applications.
Redox-Responsive Resilin-Like Hydrogels for Tissue Engineering and Drug Delivery Applications.
复制标题
DOI：
10.1002/mabi.201900122
复制
发表时间：
2019-06
影响因子：
4.6
通讯作者：
R. Su;Richard J. Galas;Charng-Yu Lin;Julie C. Liu
中科院分区：
文献类型：
--
作者：
R. Su;Richard J. Galas;Charng-Yu Lin;Julie C. Liu
Resilin是一种存在于昆虫幼虫中的蛋白质，以其出色的弹性特性而闻名。作者的实验室先前开发了一种重组蛋白，该蛋白由冈比亚按蚊的共有节枝弹性蛋白样重复序列组成，并证明了其在软骨和血管工程中的潜力。为了拓宽节枝弹性蛋白样蛋白的多功能性，本研究利用含有二硫键的可裂解交联剂来开发具有氧化还原响应性的智能节枝弹性蛋白样水凝胶。该水凝胶具有多孔结构和稳定的储能模量（G '），为0.3kPa。在水凝胶上培养24小时的NIH/3 T3成纤维细胞具有高存活率（>95%）。此外，氧化还原响应性水凝胶在还原性环境（10 mm谷胱甘肽（GSH））中显示出显著的降解。在体外评估包封在水凝胶内的荧光标记的葡聚糖的释放曲线。对于估计大于凝胶筛目尺寸的葡聚糖，由于水凝胶网络的降解，在还原剂存在下观察到更快的释放。因此，这些研究表明，这些智能水凝胶在各种应用中的潜力，从组织工程的支架到靶向肿瘤细胞内还原环境的药物递送系统。
Resilin, a protein found in insect cuticles, is renowned for its outstanding elastomeric properties. The authors' laboratory previously developed a recombinant protein, which consisted of consensus resilin-like repeats from Anopheles gambiae, and demonstrated its potential in cartilage and vascular engineering. To broaden the versatility of the resilin-like protein, this study utilizes a cleavable crosslinker, which contains a disulfide bond, to develop smart resilin-like hydrogels that are redox-responsive. The hydrogels exhibit a porous structure and a stable storage modulus (G') of ≈3 kPa. NIH/3T3 fibroblasts cultured on hydrogels for 24 h have a high viability (>95%). In addition, the redox-responsive hydrogels show significant degradation in a reducing environment (10 mm glutathione (GSH)). The release profiles of fluorescently labeled dextrans encapsulated within the hydrogels are assessed in vitro. For dextran that is estimated to be larger than the mesh size of the gel, faster release is observed in the presence of reducing agents due to degradation of the hydrogel networks. These studies thus demonstrate the potential of using these smart hydrogels in a variety of applications ranging from scaffolds for tissue engineering to drug delivery systems that target the intracellular reductive environments of tumors.