Suspension cell culture and in vivo and in vitro chromosome constitution of mouse leukemia L1210.
Suspension cell culture and in vivo and in vitro chromosome constitution of mouse leukemia L1210.
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小鼠白血病L1210的悬浮细胞培养及体内外染色体组成。
DOI：
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发表时间：
1966
期刊：
影响因子：
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通讯作者：
K. Ulrich
中科院分区：
文献类型：
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作者：
George E. Moore;A. Sandberg;K. Ulrich
建立了小鼠白血病 L1210 的体外培养物，以及悬浮培养物的最佳生长条件。与体外其他细胞系相比，L1210 显示出对叶酸的较高需求。体外培养的 DBA/2 小鼠仅需 10 个活细胞即可死亡。腹水细胞显示出 40 条染色体的清晰模式，其中有一条微小的标记染色体和一条超小的 T 型（端着丝粒）染色体。体外培养的细胞呈40或41条染色体模式，含有微小标记，但不含有超小染色体；此外，在培养的细胞中观察到m型（偏着丝粒）标记染色体。这种体外系统应该有助于评估与致癌作用、药物作用和细胞遗传学动力学相关的核发生，以及营养和代谢研究。
An in vitro culture of mouse leukemia L1210 was established, along with conditions for optimal growth in suspension culture. L1210 showed a high requirement for folic acid in comparison with other cell lines in vitro. As few as 10 viable cells cultured in vitro killed DBA/2 mice. Cells from ascites showed a sharp mode of 40 chromosomes, with a minute marker chromosome and an extra-small, T-type (telocentric) chromosome. Cells cultured in vitro showed a mode of 40 or 41 chromosomes and contained the minute marker but not the extra-small chromosome; in addition, an m-type (metacentric) marker chromosome was observed in the cultured cells. This in vitro system should lend itself to evaluation of karyogenesis in relation to carcinogenesis, drug effects, and cytogenetic dynamics, as well as to nutritional and metabolic studies.