MEIOSIS IN OVARIAN OOCYTES OF ADULT MAMMALS
MEIOSIS IN OVARIAN OOCYTES OF ADULT MAMMALS
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DOI：
10.1038/196446a0
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发表时间：
1962-01-01
期刊：
影响因子：
64.8
通讯作者：
EDWARDS, RG
中科院分区：
文献类型：
--
作者：
EDWARDS, RG
建议在比双线期晚的减数分裂阶段获得卵母细胞的方法，双线期通常在出生前或出生后不久达到。一种在某些物种中被证明是成功的方法（例如，小鼠、大鼠和仓鼠）是通过刺穿卵泡将卵母细胞释放到培养基中，之后许多卵母细胞立即恢复其成熟。看来是卵泡内的抑制作用而不是卵母细胞中的一个因素导致了网状细胞阶段的持续，卵泡的破裂使卵母细胞从抑制剂中释放出来。灵长类动物和狗的卵母细胞对这项技术的反应不那么好，并指出了可能的替代方案。
Methods are suggested for obtaining oocytes at meiotic stages later than diplotene, which is usually reached just before or shortly after birth. One method which has proved successful in some species (e.g., mouse, rat and hamster) is to liberate the oocytes into a culture medium by puncturing the follicles, after which many oocytes immediately resume their maturation. It appears that an inhibitory influence within the follicle rather than a factor in the oocyte causes the dictyate stage to persist, and rupture of the follicle releases the oocyte from the inhibitor. Primate and dog oocytes have not responded so well to this technique, and possible alternatives are indicated.