Geometric control of cell life and death
Geometric control of cell life and death
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DOI：
10.1126/science.276.5317.1425
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发表时间：
1997-05-30
期刊：
影响因子：
56.9
通讯作者：
Ingber, DE
中科院分区：
文献类型：
--
作者：
Chen, CS;Mrksich, M;Ingber, DE
人和牛的毛细血管内皮细胞通过使用含有细胞外基质涂层的粘附岛的微图案底物从生长切换到凋亡，以逐渐限制细胞的伸展。通过改变多个焦点粘连大小的岛之间的间距，在保持细胞-基质接触总面积恒定的同时，细胞扩散也是不同的。研究发现，细胞形状决定了单个细胞的生长或死亡，而与用于调节黏附的基质蛋白或整合素抗体的类型无关。因此，细胞生长和活性的局部几何控制可能代表了组织微环境内发育调节的基本机制。
Human and bovine capillary endothelial cells were switched from growth to apoptosis by using micropatterned substrates that contained extracellular matrix-coated adhesive islands of decreasing size to progressively restrict cell extension. Cell spreading also was varied while maintaining the total cell-matrix contact area constant by changing the spacing between multiple focal adhesion-sized islands. Cell shape was found to govern whether individual cells grow or die, regardless of the type of matrix protein or antibody to integrin used to mediate adhesion. Local geometric control of cell growth and viability may therefore represent a fundamental mechanism for developmental regulation within the tissue microenvironment.