Topological analysis of the ATP-gated ionotropic [correction of ionotrophic] P2X2 receptor subunit.
Topological analysis of the ATP-gated ionotropic [correction of ionotrophic] P2X2 receptor subunit.
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ATP 门控离子型 [离子型校正] P2X2 受体亚基的拓扑分析。
DOI：
10.1016/s0014-5793(98)00179-3
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发表时间：
1998
期刊：
影响因子：
3.5
通讯作者：
Voigt,MM
中科院分区：
文献类型：
--
作者：
Torres,GE;Egan,TM;Voigt,MM
我们研究了在HEK 293细胞中表达的P2X2受体亚单位的跨膜拓扑结构。利用串联表达的两个P2X亚基进行的初步研究表明，氨基和羧基位于膜的同一侧。免疫荧光研究表明，氨基和羧基末端在细胞质中定位。最后，N-糖基化扫描突变显示，插入到中心环中的报告位点，而不是那些在氨基或羧基末端区域的报告位点，是糖基化的，因此表明该结构域位于细胞外。我们的结果支持细胞内带有氨基和羧基的P2X受体的两个跨膜排列。
We investigated the transmembrane topology of the P2X2receptor subunit expressed in HEK 293 cells. Initial studies using two P2X subunits expressed in tandem indicated that the amino‐ and carboxy‐termini are on the same side of the membrane. Immunofluorescence studies showed the cytoplasmic orientation of the amino‐ and carboxy‐termini. Finally,N‐glycosylation scanning mutagenesis revealed that reporter sites inserted into the central loop, but not those in the amino‐ or carboxy‐terminal regions, were glycosylated, thus suggesting an extracellular placement for that domain. Our results support a two‐transmembrane arrangement for P2X receptors with intracellular amino‐ and carboxy‐termini.