Point-spread function engineering to reduce the impact of spherical aberration on 3D computational fluorescence microscopy imaging.
Point-spread function engineering to reduce the impact of spherical aberration on 3D computational fluorescence microscopy imaging.
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DOI：
10.1364/oe.19.023298
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发表时间：
2011-11
期刊：
影响因子：
3.8
通讯作者：
Shuai Yuan;C. Preza
中科院分区：
文献类型：
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作者：
Shuai Yuan;C. Preza
研究了不同立方相位掩模设计的波前编码（WFE）在工程化三维点扩散函数（PSF）中的应用，以降低PSF对深度引起的球面像差（SA）的敏感性，从而影响三维显微成像的计算复杂度。WFE-PSF的散焦和SA的灵敏度进行了评估，作为相位掩模参数的函数，使用均方误差度量，以方便选择扩展景深（EDOF）显微镜和计算光学切片显微镜（COSM）的掩模设计。对光瞳相位贡献和模拟WFE显微镜图像的进一步研究评估了工程化的PSF，并证明了在30 μm的样品深度上SA不敏感。尽管其对SA的灵敏度低，但用于COSM的成功WFE设计保持了对散焦的高灵敏度，因为这是光学切片所期望的。
Wavefront encoding (WFE) with different cubic phase mask designs was investigated in engineering 3D point-spread functions (PSF) to reduce their sensitivity to depth-induced spherical aberration (SA) which affects computational complexity in 3D microscopy imaging. The sensitivity of WFE-PSFs to defocus and to SA was evaluated as a function of phase mask parameters using mean-square-error metrics to facilitate the selection of mask designs for extended-depth-of-field (EDOF) microscopy and for computational optical sectioning microscopy (COSM). Further studies on pupil phase contribution and simulated WFE-microscope images evaluated the engineered PSFs and demonstrated SA insensitivity over sample depths of 30 μm. Despite its low sensitivity to SA, the successful WFE design for COSM maintains a high sensitivity to defocus as it is desired for optical sectioning.