Nanobody-alkaline phosphatase fusion-mediated phosphate-triggered fluorescence immunoassay for ochratoxin a detection
Nanobody-alkaline phosphatase fusion-mediated phosphate-triggered fluorescence immunoassay for ochratoxin a detection
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纳米抗体-碱性磷酸酶融合介导的磷酸盐触发荧光免疫分析法检测赭曲霉毒素a
DOI：
10.1016/j.saa.2019.117617
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发表时间：
2020-02-05
影响因子：
4.4
通讯作者：
Liu, Xing
中科院分区：
文献类型：
--
作者：
Wang, Xuerou;Wang, Yuanyuan;Liu, Xing
赭曲霉毒素A（OTA）是一种严重危害人类和动物健康的霉菌毒素。在本研究中，开发了一种纳米抗体 - 碱性磷酸酶融合介导的磷酸触发荧光免疫分析方法（Nb - AP介导的PT - FIA）用于检测OTA。基于构建的用于Nb - AP的磷酸触发荧光传感系统以及最佳工作条件，Nb - AP介导的PT - FIA的半数最大抑制浓度（IC50）为0.46纳克/毫升，检测限（IC10）为0.12纳克/毫升，线性范围（IC20 - 80）分别为0.2 - 1.26纳克/毫升。回收实验表明Nb - AP介导的PT - FIA具有可接受的准确度和精密度，并且该结果通过带有荧光检测器的高效液相色谱法进行了验证。因此，这种提出的方法适用于食品和环境中OTA以及其他低分子量有毒分析物的灵敏、快速且低成本的检测。（C）2019爱思唯尔有限公司。保留所有权利。
Ochratoxin A (OTA) is a kind of mycotoxin that seriously harms the health of humans and animals. In this study, a nanobody-alkaline phosphatase fusion-mediated phosphate-triggered fluorescence immunoassay (Nb-AP-mediated PT-FIA) was developed for detecting OTA. Based on the constructed phosphate-triggered fluorescence sensing system for Nb-AP and the optimal working conditions, the Nb-AP-mediated PT-FIA has a half maximal inhibition concentration (IC50) of 0.46 ng/mL, a limit of detection (IC10) of 0.12 ng/mL, and a linear range (IC20-80) of 02-1.26 ng/ml, respectively. The recovery experiment indicated acceptable accuracy and precision of the Nb-AP-mediated PT-FIA, and the results were validated by high performance liquid chromatography with fluorescence detector. Thus this proposed method is applicable to sensitive, rapid, and low-cost detection of OTA and other toxic analytes with low molecular weight in food and environment. (C) 2019 Elsevier B.V. All rights reserved.