Effects of exercise intensity and environmental stress on indices of oxidative stress and iron homeostasis during exercise in the horse
Effects of exercise intensity and environmental stress on indices of oxidative stress and iron homeostasis during exercise in the horse
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DOI：
10.1007/bf00376495
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发表时间：
1996-08-01
期刊：
影响因子：
--
通讯作者：
Marlin, DJ
中科院分区：
文献类型：
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作者：
Mills, PC;Smith, NC;Marlin, DJ
在凉爽[20 ℃，40 ℃相对湿度（RH）]或炎热/潮湿（30 ℃，80%RHS）环境条件下，比较了6匹健康马长期可变强度和短期高强度运动对氧化应激和铁稳态指数的影响。运动方案旨在模拟马的竞争，包括赛跑（激烈的运动）或为期3天的比赛（长时间运动）的速度和耐力阶段。增加的脂质过氧化氢和氧化谷胱甘肽（GSSG）的溶血产物浓度的血浆浓度在30分钟内完成的运动，表明生产的活性氧（ROS）和脂质膜过氧化。在高温和高湿度下，马无法完成长时间运动方案。与高强度[105.2（14.9）mu M和63.6（8.6）mu M]相比，这与脂质氢过氧化物[138.2（17.7）mu M和GSSG [110.6（18.2）mu M]的较高最大值相一致。[分别为100.7（187）μ M和86.2（9.1）μ M]或在较冷的环境条件下进行的长时间运动。在湿热环境条件下，运动最后阶段的持续时间与脂质过氧化氢（r=0.85）、GSSG（r=0.94）、黄嘌呤（r=0.92）和尿酸（r=0.96）水平的升高之间存在显著相关性。运动还降低了血浆中的铁（Fe）结合抗氧化活性，并增加了血浆总铁水平，尽管这只对环境条件下的长时间驱魔有意义。在运动的任何阶段，血浆中均未检测到游离Fe，运动后24 h内，其他生化参数的变化均恢复至运动前水平。结果表明，运动可诱导健康马的生化参数发生变化，这些生化参数指示氧化应激，并且在高温高湿条件下运动时，这种变化加剧。
The effects of prolonged variable-intensity and short-term high-intensity exercise on indices of oxidative stress and iron homeostasis were compared in six fit horses under cool [20 degrees C, 40 degrees C relative humidity (RH)] or hot/humid (30 degrees C, 80% RHS environmental conditions. The exercise protocols were designed to simulate equine competition, including racing (intense exercise) or the speed and endurance phase of a 3-day event (prolonged exercise). Increased plasma concentrations of lipid hydroperoxides and haemolysate concentrations of oxidised glutathione (GSSG) were measured within 30 min of the completion of exercise, indicating production of reactive oxygen species (ROS) and lipid membrane peroxidation. The horses were unable to complete the prolonged exercise protocol at high temperature and humidity. This coincided with higher maximal values of lipid hydroperoxides [138.2 (17.7) mu M and GSSG [110.6 (18.2) mu M], compared to high-intensity [105.2 (14.9) mu M and 63.6 (8.6) mu M. respectively] or prolonged [100.7 (187) mu M and 86.2 (9.1) mu M, respectively] exercise performed under cooler environmental conditions. Significant correlations were found between the duration of the final stage of exercise during hot/humid environmental conditions and increased levels of lipid hydroperoxides (r=0.85), GSSG (r=0.94), xanthine (r=0.92) and uric acid (r=0.96). Excerise also decreased the iron (Fe)-binding antioxidant activity of the plasma and increased the total plasma Fe levels, although this was only significant for prolonged exorcise in ambient conditions. There was no detectable free Fe in the plasma at any stage of exercise, Other changes in biochemical parameters had returned to pre-exercise levels within 24 h after exercise, The results show that exercise can induce changes in biochemical parameters that are indicative of oxidative stress in the fit horse and that this was, exacerbated during exercise at high temperature and humidity.