INTRACELLULAR CHLORIDE ACTIVITY IN RABBIT PAPILLARY-MUSCLE - EFFECT OF OUABAIN
INTRACELLULAR CHLORIDE ACTIVITY IN RABBIT PAPILLARY-MUSCLE - EFFECT OF OUABAIN
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DOI：
10.1152/ajpcell.1981.240.5.c183
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发表时间：
1981-01-01
影响因子：
--
通讯作者：
SCHANNE, OF
中科院分区：
文献类型：
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作者：
CAILLE, JP;RUIZCERETTI, E;SCHANNE, OF
细胞内氯活度。**图表**。在体外条件下测定兔乳头肌的膜电位(Vm)。细胞内氯离子浓度(Cli)通过测量总含水量、胞外间隙和总氯离子浓度来估算。检测治疗性浓度(10-8M)和毒性浓度(10-6M)哇巴因对上述参数的影响。氯气敏感微电极为液体离子交换型。在计算**图形**时考虑了HCO3-的选择性。在使用两种不同方案的11个对照实验中。**图表**。在心内膜下和更深层的细胞中被检测到。平均Vm分别为-78.7和-78.0 mV，平均胞浆Cl-活度分别为17.5和17.7 mM。氯平衡电位分别为-43.5 mV和-43.2 mV。CL-在兔乳头状肌中不呈被动分布。哇巴因(10-8M)不改变Vm或.**图形**。在哇巴因中毒浓度下，Vm在浅层细胞降至-68.0 mV，在深层细胞降至-67.8 mV，但.**图形**。保持不变。在体外条件下，细胞内的氯离子明显分布在一个以上的细胞室中。
Intracellular chloride activity .**GRAPHIC**. and membrane potential (Vm) were measured in rabbit papillary muscle under in vitro conditions. The cellular Cl- concentration (Cli) was estimated from measurements of total water content, extracellular space and total Cl- concentration. The effects of therapeutic (10-8 M) and toxic (10-6 M) concentrations of ouabain on these parameters were tested. The Cl- sensitive microelectrodes were of the liquid-ion exchanger type. Selectivity for HCO3- was taken into account in the calculation of .**GRAPHIC**. In 11 control experiments made with 2 different protocols .**GRAPHIC**. was determined in subendocardial and in deeper cells. The mean Vm were -78.7 and -78.0 mV and the mean cytoplasmic Cl- activities were 17.5 and 17.7 mM, respectively. The Cl- equilibrium potentials were -43.5 and -43.2 mV. Cl- is not passively distributed in rabbit papillary muscle. Ouabain (10-8 M) did not change Vm or .**GRAPHIC**. At a toxic concentration of ouabain, Vm fell to -68.0 mV in superficial cells and to -67.8 mV in deeper cells, but .**GRAPHIC**. remained unchanged. Under in vitro conditions intracellular Cl- apparently is distributed within more than 1 cellular compartment.