POLYPEPTIDES OF RAT-LIVER MITOCHONDRIA - IDENTIFICATION OF A 36,000 DALTON POLYPEPTIDE AS SUBUNIT OF ORNITHINE TRANSCARBAMYLASE
POLYPEPTIDES OF RAT-LIVER MITOCHONDRIA - IDENTIFICATION OF A 36,000 DALTON POLYPEPTIDE AS SUBUNIT OF ORNITHINE TRANSCARBAMYLASE
复制标题
DOI：
10.1016/0006-291x(76)90769-5
复制
发表时间：
1976-01-01
影响因子：
3.1
通讯作者：
CLARKE, S
中科院分区：
文献类型：
--
作者：
CLARKE, S
大鼠肝线粒体进行十二烷基硫酸盐凝胶电泳后观察到的主要多肽条带之一是MW为36，000道尔顿的组分，占总线粒体蛋白的3-4%。这条带，命名为Va（1），用尿素循环的一种酶，即鸟氨酸转氨甲酰酶（EC 2.1.3.3）纯化。证据表明，带Va多肽是一个单一的分子物种对应于这种酶的多肽链。
One of the major polypeptide bands seen after rat liver mitochondria are subjected to dodecyl sulfate gel electrophoresis is a component with a MW of 36,000 daltons that makes up 3-4% of the total mitochondrial protein. This band, designated Va (1), purifies with an enzyme of the urea cycle, ornithine transcarbamylase (EC 2.1.3.3). Evidence is presented that the band Va polypeptide is a single molecular species corresponding to the polypeptide chain of this enzyme.