Systematic delineation of scan modes in multidimensional mass spectrometry.
Systematic delineation of scan modes in multidimensional mass spectrometry.
复制标题
多维质谱中扫描模式的系统描述。
DOI：
10.1021/ac00216a016
复制
发表时间：
1990
影响因子：
7.4
通讯作者：
Cooks,RG
中科院分区：
文献类型：
--
作者：
Schwartz,JC;Wade,AP;Enke,CG;Cooks,RG
总共有2”个具有1或0质量维度的实验，沿着增加了具有更高质量维度的实验数量。还存在一组2n个基本扫描模式，即，只规定单个离子的质荷比而不规定它们的相互关系的实验。定义离子质量之间函数关系的扫描（例如，中性丢失扫描）将复杂性引入MSn实验中可用的扫描类型总数，分别给出0到5阶的总共1、2、5、15、52和203个实验。结果表明，低阶实验数据的组合可以用来构造高阶谱。本文还演示了从高维数据中提取低质量维数数据的方法。本文还介绍了一种不同的降维方法，即把分散的数据投影到更少的质量维中。分析揭示了几种新类型的扫描模式，包括具有单位质量维度的MS/MS/MS扫描，称为选择性中性丢失扫描，以及几种新的MS/MS/MS扫描，其是二维的In质量。提供了这些新实验的实例，并讨论了它们的潜在价值。
Is a total of 2" experiments that have 1 or 0 mass dimensions, along with an Increasing number of experiments of higher mass dimensionality. There also exists a set of 2n fundamental scan modes, viz., experiments In which only mass-to-charge ratios of individual ions, but not their Interrelation-ships, are specified. Scans In which functional relationships between Ion masses are defined (eg, neutral loss scans) Introduce complexity Into the total number of scan types available In an MSn experiment, giving a total of 1, 2, 5, 15, 52, and 203 experiments of 0 through 5th order, respectively. It Is shown that combinations of data from lower order ex-periments can be used to construct higher order spectra. Extraction of data of lower mass dimensionality from data of higher dimensionality Is also demonstrated. A different me-thod of reducing dimensionality, projection of dispersed data back into a smaller number of mass dimensions, Is also In-troduced and characterized. The analysis reveals several new types of scan modes Including an MS/MS/MS scan having unit mass dimensionality, referred to as the selective neutral-loss scan, and several new MS/MS/MS scans that are two-dimensional In mass. Examples of these new experiments are provided, and their potential value Is discussed.