Immunocytochemical detection of the onco-developmental protein oncomodulin in pre-neoplastic and neoplastic hepatocellular lesions during hepatocarcinogenesis in rats.
Immunocytochemical detection of the onco-developmental protein oncomodulin in pre-neoplastic and neoplastic hepatocellular lesions during hepatocarcinogenesis in rats.
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大鼠肝癌发生过程中肿瘤前期和肿瘤性肝细胞病变中肿瘤发育蛋白癌调节蛋白的免疫细胞化学检测。
DOI：
10.1002/ijc.2910430430
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发表时间：
1989
影响因子：
6.4
通讯作者：
Galand,P
中科院分区：
文献类型：
--
作者：
Bernaert,D;Brewer,LM;MacManus,JP;Galand,P
癌调节蛋白是一种钙结合蛋白，在胚胎外人类和大鼠胎盘细胞以及各种肿瘤中可检测到，但在任何正常胚胎或成年啮齿动物或人类组织中均不可检测到。它也不存在于增殖活性的胎鼠或再生的成年大鼠肝脏中。使用纯化的癌调节蛋白抗体，在DENA治疗诱导的大鼠肝癌发生过程中，研究了癌前病变和肿瘤性肝脏病变中是否存在这种肿瘤发育标志物。在改变的肝细胞灶、肿瘤结节和HC中观察到阳性免疫染色，但在组织学正常的周围肝实质中未观察到。肿瘤调节蛋白阳性病灶的比例从DENA治疗后2 - 3个月的20 - 25%逐渐上升到6个月及以后的约88%。阳性肿瘤结节的比例从5个月时的50%增加到9个月及以后的约73%（范围36 - 100）; DENA治疗后10 - 20个月发现的HC中有88%也是阳性的。早期肿瘤结节的肿瘤调节蛋白阳性比例（50%）与相同治疗持续时间后的病灶相似，这与它们之间的谱系关系一致，但肿瘤调节蛋白表达不太可能影响病灶结节的转变。肿瘤调节蛋白在恶性进展中的作用（如果有的话）仍有待阐明。这似乎是不可能的，它是一个简单的相关性增殖活动。
Oncomodulin is a calcium‐binding protein, detectable in extra‐embryonic human and rat placental cells and in a wide variety of tumors, but not in any normal embryonic or adult rodent or human tissues. It is also absent from proliferatively active fetal or regenerating adult rat liver. The presence of this oncodevelopmental marker was investigated in preneoplastic and neoplastic liver lesions during hepatocarcinogenesis induced in rats by DENA treatment, using an antibody raised against purified oncomodulin. Positive immunostaining was observed in foci of altered hepatocytes, in neoplastic nodules and in HC, but not in the histologically normal surrounding liver parenchyma. The proportion of oncomodulin‐positive foci gradually rose from 20–25% at 2–3 months after DENA treatment, to about 88% at 6 months and later. The proportion of positive neoplastic nodules increased from 50% at 5 months to about 73% (range 36–100) at 9 months and later; 88% of the HC found 10 to 20 months after DENA treatment were also positive. That early neoplastic nodules are oncomodulin‐positive in a proportion (50%) similar to that of foci after the same duration of treatment is consistent with a lineage relationship between them but makes it unlikely that oncomodulin expression conditions the focusnodule transition. The role, if any, of oncomodulin in malignant progression remains to be elucidated. It seems out of the question that it is a simple correlate of proliferative activity.