Oxford Nanopore Technology: Revolutionizing Genomics Research in Plants
Oxford Nanopore Technology: Revolutionizing Genomics Research in Plants
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牛津纳米孔技术：彻底改变植物基因组学研究
DOI：
10.1016/j.tplants.2021.11.004
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发表时间：
2021
影响因子：
20.5
通讯作者：
Huang Cheng
中科院分区：
文献类型：
--
作者：
Xu Ying（徐莹）;Luo Hongbing;Wang Zhili;Lam Hon-Ming;Huang Cheng
牛津纳米孔技术（ONT）是最有前途的长读长技术之一，能够产生超长读长，可以克服短读长遇到的问题，例如表征结构变异和高度重复的区域。 ONT 通过检测与穿过纳米孔的每个核苷酸的特征相对应的离子电流信号的变化来记录天然 DNA 和 RNA 的各种碱基和修饰。 ONT 使研究人员能够以前所未有的分辨率探索植物基因组、转录组和表观基因组。
Oxford Nanopore Technology (ONT) is one of the most promising long-read technologies able to produce ultra-long reads, which can overcome issues encountered with short reads, such as characterizing structural variants and highly repeated regions. ONT documents various bases and modifications of native DNA and RNA by detecting the changes in ionic current signals corresponding to the characteristics of each nucleotide passing through a nanopore. ONT enables researchers to explore plant genomes, transcriptomes, and epigenomes at an unprecedented resolution.