Transdermal photopolymerization for minimally invasive implantation
Transdermal photopolymerization for minimally invasive implantation
复制标题
DOI：
10.1073/pnas.96.6.3104
复制
发表时间：
1999-03-16
影响因子：
11.1
通讯作者：
Langer, R
中科院分区：
文献类型：
--
作者：
Elisseeff, J;Anseth, K;Langer, R
光聚合广泛用于医学中以产生用于诸如骨重建和用于人工植入物的涂层的应用的聚合物网络。这些光聚合通过在“开放”环境（如口腔）中或在侵入性程序（如手术）期间将材料直接暴露于光而发生。我们假设穿透组织（包括胫骨）的光可间接引起光聚合。然后可以将液体材料s.c.并通过将皮肤的外表面暴露于光而固化。为了验证这一假设，研究了UVA和可见光通过皮肤的渗透。建模预测了经皮聚合的可行性，其中仅需要2分钟的曝光来光聚合人胫骨下的植入物。为了建立这些建模研究的有效性，首先通过使用“可注射”软骨作为模型系统将经皮聚合应用于组织工程。将聚合物/软骨细胞构建体皮下、皮下和经皮注射。光聚合的在第2、4和7周收获的植入物证明了胶原蛋白和蛋白聚糖的产生以及具有与天然新软骨相当的组织结构的组织学。为了进一步检查这种现象并测试透皮光聚合用于药物释放装置的适用性，将白蛋白（一种模型蛋白）从光聚合水凝胶中释放1周。随着进一步的研究，经皮光聚合可能用于创造各种新的微创外科手术，应用范围从整形外科和矫形外科到组织工程和药物输送。
Photopolymerizations are widely used in medicine to create polymer networks for use in applications such as bone restorations and coatings for artificial implants. These photopolymerizations occur by directly exposing materials to light in "open" environments such as the oral cavity or during invasive procedures such as surgery, We hypothesized that light, which penetrates tissue including shin, could cause a photopolymerization indirectly. Liquid materials then could be injected s.c. and solidified by exposing the exterior surface of the skin to light. To test this hypothesis, the penetration of UVA and visible light through skin was studied, Modeling predicted the feasibility of transdermal polymerization with only 2 min of light exposure required to photopolymerize an implant underneath human shin, To establish the validity of these modeling studies, transdermal photopolimerization first was applied to tissue engineering by using "injectable" cartilage as a model system. Polymer/chondrocyte constructs were injected s,c, and transdermally. photopolymerized. Implants harvested at 2, 4, and 7 weeks demonstrated collagen and proteoglycan production and histology with tissue structure comparable to native neocartilage, To further examine this phenomenon and test the applicability of transdermal photopolymerization for drug release devices, albumin, a model protein, was released for 1 week from photopolymerized hydrogels. With further study, transdermal photpolymerization potentially could be used to create a variety of new, minimally invasive surgical procedures in applications ranging from plastic and orthopedic surgerSr to tissue engineering and drug delivery.