High levels of a heat-labile calmodulin-binding protein (CaM-BP80) in bovine neostriatum.
High levels of a heat-labile calmodulin-binding protein (CaM-BP80) in bovine neostriatum.
复制标题
牛新纹状体中高水平的不耐热钙调蛋白结合蛋白 (CaM-BP80)。
DOI：
10.1021/bi00550a016
复制
发表时间：
1980
期刊：
影响因子：
2.9
通讯作者：
Wai Yiu Cheung
中科院分区：
文献类型：
--
作者：
Wallace Rw;Tallant Ea;Wai Yiu Cheung
牛脑中含有一种热不稳定的、80，000-道尔顿的钙调素结合蛋白（CaM-BP 80），它在体外抑制钙调素依赖的环3 '，5'-核苷酸磷酸二酯酶、腺苷酸环化酶和Ca ~（2+）-ATP酶的活性。CaM-BP 80由两种多肽（60，000和18，500道尔顿）组成，以1：1的比例存在。在兔中产生针对CaM-BP 80的抗体，并开发放射免疫测定法，其具有60 fmol CaM-BP 80的灵敏度。用放射免疫法测定了牛不同组织中CaM-BP 80的含量。这种蛋白质主要在大脑中发现，在新纹状体中的含量特别高。这些结果，连同突触后致密物和突触后树突的微管处的CaM-BP 80的免疫组织化学定位[Wood，J.G.，华莱士河，Whitaker，J.，& Cheung，W.Y.（1980）J. Cell Biol.84，66-76]，表明该蛋白质可能在大脑中的神经递质作用位点和微管功能水平上起作用。
Bovine brain contains a heat-labile, 80,000-dalton calmodulin-binding protein (CaM-BP80) which inhibits the calmodulin-dependent activities of cyclic 3',5'-nucleotide phosphodiesterase, adenylate cyclase, and Ca2+-ATPase in vitro. CaM-BP80 is composed of two polypeptides (60,000 and 18,500 daltons) present in a 1:1 ratio. An antibody directed against CaM-BP80 was raised in rabbits, and a radioimmunoassay was developed, having a sensitivity of 60 fmol of CaM-BP80. Using the radioimmunoassay, we determined the levels of CaM-BP80 in various bovine tissues. The protein was found primarily in the brain, present in particularly high levels in the neostriatum. These results, together with immunohistochemical localization of CaM-BP80 at the postsynaptic densities and the microtubules of postsynaptic dendrites [Wood, J.G., Wallace, R., Whitaker, J., & Cheung, W.Y. (1980) J. Cell Biol. 84, 66-76], suggest that the protein may have a role in the cerebrum at the site of neurotransmitter action and at the level of microtubular function.