TIEG1-NULL OSTEOCYTES DISPLAY DEFECTS IN THEIR MORPHOLOGY, DENSITY AND SURROUNDING BONE MATRIX.
TIEG1-NULL OSTEOCYTES DISPLAY DEFECTS IN THEIR MORPHOLOGY, DENSITY AND SURROUNDING BONE MATRIX.
复制标题
DOI：
10.1142/s0218957709002304
复制
发表时间：
2009-09
影响因子：
--
通讯作者：
Bensamoun SF
中科院分区：
文献类型：
--
作者：
Haddad O;Hawse JR;Subramaniam M;Spelsberg TC;Bensamoun SF
通过培育 TGFβ 诱导的早期基因 1 (TIEG1) 敲除 (KO) 小鼠，我们证明 TIEG1 在成骨细胞介导的骨矿化以及骨对机械应变的抵抗力中发挥重要作用。为了进一步研究 TIEG1 在骨骼维持中的影响，使用 TIEG1 KO 和 1、3 和 8 个月龄野生型小鼠股骨通过透射电子显微镜分析骨细胞。结果揭示了骨细胞表面和密度的年龄依赖性变化，表明 TIEG1 在骨细胞发育中的作用。此外，与野生型对照相比，TIEG1 KO 小鼠骨细胞周围矿化不足的骨基质数量有所减少。虽然人们对紧邻骨细胞的矿化不足的骨基质的功能或重要性知之甚少，但这项研究揭示了这种骨微环境的显着差异，并表明在缺乏 TIEG1 表达的情况下骨细胞功能可能会受到损害。
Through the development of TGFβ-inducible early gene-1 (TIEG1) knockout (KO) mice, we have demonstrated that TIEG1 plays an important role in osteoblast-mediated bone mineralization, and in bone resistance to mechanical strain. To further investigate the influence of TIEG1 in skeletal maintenance, osteocytes were analyzed by transmission electron microscopy using TIEG1 KO and wild-type mouse femurs at one, three and eight months of age. The results revealed an age-dependent change in osteocyte surface and density, suggesting a role for TIEG1 in osteocyte development. Moreover, there was a decrease in the amount of hypomineralized bone matrix surrounding the osteocytes in TIEG1 KO mice relative to wild-type controls. While little is known about the function or importance of this hypomineralized bone matrix immediately adjacent to osteocytes, this study reveals significant differences in this bone microenvironment and suggests that osteocyte function may be compromised in the absence of TIEG1 expression.