Quantifying Noise in Optical Tweezers by Allan Variance
Quantifying Noise in Optical Tweezers by Allan Variance
复制标题
DOI：
10.1364/oe.17.013255
复制
发表时间：
2009-07-20
期刊：
影响因子：
3.8
通讯作者：
Oddershede, Lene B.
中科院分区：
文献类型：
--
作者：
Czerwinski, Fabian;Richardson, Andrew C.;Oddershede, Lene B.
在光镊实验中，由于噪声和漂移会掩盖光镊的基本特性，因此，人们一直在努力减小噪声。例如，在一个实施例中，单分子测定。已采取各种措施来减少或消除噪音，但很难量化其效果。我们建议使用Allan方差作为一种简单而有效的方法来量化光镊设置中的噪声。我们应用该方法来确定最佳的测量时间，频率和检测方案，并在实验室中量化的声学噪声的影响。该方法也可以用于确定运行实验的最佳参数。(C)2009年美国光学学会
Much effort is put into minimizing noise in optical tweezers experiments because noise and drift can mask fundamental behaviours of, e. g., single molecule assays. Various initiatives have been taken to reduce or eliminate noise but it has been difficult to quantify their effect. We propose to use Allan variance as a simple and efficient method to quantify noise in optical tweezers setups. We apply the method to determine the optimal measurement time, frequency, and detection scheme, and quantify the effect of acoustic noise in the lab. The method can also be used on-the-fly for determining optimal parameters of running experiments. (C) 2009 Optical Society of America