Insights into insulin-like peptide system in invertebrates from studies on IGF binding domain-containing proteins in the female mud crab, Scylla paramamosain
Insights into insulin-like peptide system in invertebrates from studies on IGF binding domain-containing proteins in the female mud crab, Scylla paramamosain
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通过对雌性泥蟹（Scylla paramamosain）中含有 IGF 结合域的蛋白质的研究，深入了解无脊椎动物中的胰岛素样肽系统
DOI：
10.1016/j.mce.2015.08.019
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发表时间：
2015-11-15
影响因子：
4.1
通讯作者：
Huang, Huiyang
中科院分区：
文献类型：
--
作者：
Huang, Xiaoshuai;Ye, Haihui;Huang, Huiyang
胰岛素样肽(ILPS)广泛存在于无脊椎动物体内，在调节生长、代谢和生殖等方面发挥着重要作用。ILP信号系统在昆虫中已经得到了很好的定义，所有的关键成分都与脊椎动物的IGF信号同源，但由于缺乏相关方面的研究，脊椎动物中的IGF结合蛋白(IGFBPs)的对应蛋白并未包括在该系统中。本研究首次报道了三种无脊椎动物胰岛素样生长因子结合区(IB)蛋白基因在青蟹体内的鉴定。基因表达分析表明，它们可能与卵巢发育密切相关，但具有不同的作用。随后的生物信息学分析和体外实验表明，它们很可能是无脊椎动物内源性ILP特异性结合蛋白。更重要的是，根据目前的证据，我们推测在无脊椎动物中，ILP系统可能取代脊椎动物中的IGF系统。(C)2015爱思唯尔爱尔兰有限公司。保留所有权利。
Insulin-like peptides (ILPs) have been proved to exist extensively in invertebrates and play critical roles in regulating growth, metabolism and reproduction. ILP signaling system has been well defined in insects, with all key components homologous with vertebrate IGF signaling; however, counterparts of IGF binding proteins (IGFBPs) in vertebrates are not included in this system because of lacking sufficient researches in the related aspect. The present study firstly reports the identification of three kinds of invertebrate IGF binding (IB) domain-containing protein genes from the mud crab Scylla paramamosain. Gene expression analysis suggested that they might be closely involved in ovarian development, but with separate roles. Subsequent bioinformatics analysis and in vitro experiments indicated that they are likely to serve as endogenous ILP-specific binding proteins in invertebrates. More importantly, based on the current evidence we inferred that in invertebrate, ILP system might take the place of IGF system in vertebrate species. (C) 2015 Elsevier Ireland Ltd. All rights reserved.