Effects of Placental Extract on Cell Proliferation, Type I Collagen Production, and ALP Secretion in Human Osteosarcoma Cell Line Saos-2
Effects of Placental Extract on Cell Proliferation, Type I Collagen Production, and ALP Secretion in Human Osteosarcoma Cell Line Saos-2
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DOI：
10.2485/jhtb.26.157
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发表时间：
2017-04-01
影响因子：
0.4
通讯作者：
Wang, Pao-Li
中科院分区：
文献类型：
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作者：
Imamura, Yasuhiro;Honda, Yoshitomo;Wang, Pao-Li
猪胎盘提取物（P-plasticide extract，P-plasticide）在医药和化妆品中有着广泛的应用。然而，很少有研究检查提取物对成骨细胞系Saos-2的细胞行为的影响。在这里，我们证明了P-胎盘在体外增强Saos-2的增殖、I型胶原的产生和碱性磷酸酶（ALP）的分泌。MTT法和DNA合成法检测Saos-2细胞增殖。使用酶联免疫吸附测定和ALP测定评价I型胶原蛋白的产生和ALP分泌。用/不用20、200和2000 μ g/ml的P-胎盘处理细胞24小时。我们发现，200 μ g/ml的P-胎盘显着诱导Saos-2的增殖，并增强I型胶原的产生和ALP分泌。结果表明，P-胎盘控制成骨细胞的细胞行为，导致早期骨相关生物标志物的分泌。
Porcine placenta extract (P-placenta) is widely applied in medicine and cosmetics. However, few studies have examined the effect of the extract on the cellular behavior of the osteoblastic cell line Saos-2. Here, we demonstrated that P-placenta enhances the proliferation, collagen type I production, and alkaline phosphatase (ALP) secretion of Saos-2 in vitro. Proliferation of Saos-2 was assessed by MTT and DNA synthesis assays. Type I collagen production and ALP secretion were evaluated using enzyme-linked immunosorbent assay and ALP assays. The cells were treated with/without 20, 200 and 2000 mu g/ml of P-placenta for 24 h. We found that 200 mu g/ml P-placenta significantly induced the proliferation of Saos-2 and enhanced type I collagen production and ALP secretion. The results indicate that P-placenta controls the cellular behavior of osteoblasts, resulting in the secretion of early bone-related biomarkers.