Phylogenetic analysis reveals multiple lateral transfers of adenosine-5′-phosphosulfate reductase genes among sulfate-reducing microorganisms
Phylogenetic analysis reveals multiple lateral transfers of adenosine-5′-phosphosulfate reductase genes among sulfate-reducing microorganisms
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DOI：
10.1128/jb.184.1.278-289.2002
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发表时间：
2002-01-01
影响因子：
3.2
通讯作者：
Friedrich, MW
中科院分区：
文献类型：
--
作者：
Friedrich, MW
横向基因转移影响的进化路径的关键基因参与古老的代谢性状，如硫酸呼吸，甚至比以前预期的。本研究对腺苷-5 '-磷酸硫酸（APS）还原酶进行了系统的研究。APS还原酶是硫酸盐呼吸的关键酶，存在于所有呼吸硫酸盐的原核生物中。一个新开发的PCR检测被用来扩增和序列的APS还原酶基因，apsA，从一个分类广泛的硫酸盐还原原核生物（n = 60）的片段（类似于900 bp）。所有获得的和可用的ApsA序列的比较系统发育分析表明，在该地区的序列保守性很高。然而，ApsA和165 rRNA为基础的系统发育树的比较显示拓扑不一致影响7个成员的Syntrophobacteraceae和3个成员的硝化菌科，这显然是单系与革兰氏阳性硫酸盐还原菌（SRB）。此外，嗜热嗜热弧菌islandicus和嗜热嗜热芽孢杆菌、普通嗜热芽孢杆菌和嗜热嗜热芽孢杆菌hveragerdense在δ-变形菌革兰氏阴性SRB和革兰氏阳性SRB的辐射之间清楚地分支，并且不像从165 rRNA同源性所预期的那样靠近树根。对树状拓扑结构中这些差异的最简洁的解释是apsA基因在细菌分裂中的横向转移。供体和受体谱系ApsA序列中类似的插入和缺失模式为横向基因转移提供了额外的证据。从参考菌株的子集（n = 25），异化亚硫酸盐还原酶基因（dsrAB）的片段，其最近被提出经历了多个横向基因转移（M. Klein等人，J. Bacteriol. 183：6028-6035，2001），也进行了扩增和测序。DsrAB和ApsA为基础的树的系统发育比较表明，经常参与的革兰氏阳性和嗜热SRB之间的横向基因转移事件。
Lateral gene transfer affects the evolutionary path of key genes involved in ancient metabolic traits, such as sulfate respiration, even more than previously expected. In this study, the phylogeny of the adenosine-5'-phosphosulfate (APS) reductase was analyzed. APS reductase is a key enzyme in sulfate respiration present in all sulfate-respiring prokaryotes. A newly developed PCR assay was used to amplify and sequence a fragment (similar to 900 bp) of the APS reductase gene, apsA, from a taxonomically wide range of sulfate-reducing prokaryotes (n = 60). Comparative phylogenetic analysis of all obtained and available ApsA sequences indicated a high degree of sequence conservation in the region analyzed. However, a comparison of ApsA- and 165 rRNA-based phylogenetic trees revealed topological incongruences affecting seven members of the Syntrophobacteraceae and three members of the Nitrospinaceae, which were clearly monophyletic with gram-positive sulfate-reducing bacteria (SRB). In addition, Thermodesulfovibrio islandicus and Thermodesulfobacterium thermophilum, Thermodesulfobacterium commune, and Thermodesulfobacterium hveragerdense clearly branched off between the radiation of the delta -proteobacterial gram-negative SRB and the gram-positive SRB and not close to the root of the tree as expected from 165 rRNA phylogeny. The most parsimonious explanation for these discrepancies in tree topologies is lateral transfer of apsA genes across bacterial divisions. Similar patterns of insertions and deletions in ApsA sequences of donor and recipient lineages provide additional evidence for lateral gene transfer. From a subset of reference strains (n = 25), a fragment of the dissimilatory sulfite reductase genes (dsrAB), which have recently been proposed to have undergone multiple lateral gene transfers (M. Klein et al., J. Bacteriol. 183:6028-6035, 2001), was also amplified and sequenced. Phylogenetic comparison of DsrAB- and ApsA-based trees suggests a frequent involvement of gram-positive and thermophilic SRB in lateral gene transfer events among SRB.