CHARACTERIZATION OF THE SMALL RNA OF THE BACTERIOPHAGE-PHI-29 DNA PACKAGING MACHINE
CHARACTERIZATION OF THE SMALL RNA OF THE BACTERIOPHAGE-PHI-29 DNA PACKAGING MACHINE
复制标题
DOI：
10.1093/nar/15.17.7081
复制
发表时间：
1987-09-11
影响因子：
14.9
通讯作者：
ANDERSON, D
中科院分区：
文献类型：
--
作者：
GUO, PX;BAILEY, S;ANDERSON, D
噬菌体ø29 DNA包装机的前头连接器是用在体外调节DNA包装的小RNA重建的。 120个核苷酸RNA的完整序列证明其起源于ø29 DNA左侧早期区域的启动子PE1(A1)，在组装过程中末端首先被包装。前头 RNA 在序列和组成上与真细菌 5S rRNA 明显不同。
The prohead connector of the bacteriophage ø29 DNA packaging machine was reconstructed with the small RNA that regulates DNA packagingin vitro. The complete sequence of the 120 nucleotide RNA proved its origination from the promoter PE1(A1) of the left early region of ø29 DNA, the end packaged first during assembly. The prohead RNA was clearly distinct from eubacterial 5S rRNA in sequence and composition.