Nutritional Quality and Antioxidant Activity of Wheatgrass (Triticum aestivum) Unwrap by Proteome Profiling and DPPH and FRAP assays
Nutritional Quality and Antioxidant Activity of Wheatgrass (Triticum aestivum) Unwrap by Proteome Profiling and DPPH and FRAP assays
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DOI：
10.1111/1750-3841.14224
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发表时间：
2018-08-01
影响因子：
3.9
通讯作者：
Chougale, Ashok D.
中科院分区：
文献类型：
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作者：
Parit, Santosh B.;Dawkar, Vishal V.;Chougale, Ashok D.
植物在医学领域的宝贵贡献是众所周知的。小麦（Triticum aestivum）种子和幼苗是重要的食物和饲料来源，因为它们含有多种促进健康的化合物。采用液相色谱-质谱联用技术对小麦种子萌发后0、8、16 d的蛋白质组进行了分析。共鉴定了297个蛋白质，它们的功能注释显示，其中大多数参与预防许多疾病、氧化应激、初级代谢、储存和能量相关机制。特别值得一提的是，过氧化物酶、超氧化物歧化酶和细胞色素在冰草中大量存在。在铁还原抗氧化能力测定中，与0 DAG相比，16 DAG后的抗氧化活性增加了1.55倍，但在8 DAG后其降低。DPPH法测定的植物提取物的抗氧化活性在3个时间点后均呈上升趋势。DPPH自由基清除率分别为15%、22%和30%。所获得的观察结果表明，植物的抗氧化能力与幼苗的年龄成正比。对小麦草获得的数据表明，它可以是一个强大的抗氧化剂，由于其自由基清除活性，并可用于压力和滋养人体health.Practical ApplicationWheatgrass含有矿物质，植物化学物质，活性酶和维生素，可以很容易地被吸收。小麦草汁的消费可以提供更好的健康益处。关于小麦草汁的有益特性的信息在这项工作中被清楚地提到。已知小麦草中发现的蛋白质参与预防许多疾病、氧化应激、初级代谢、储存和能量相关机制。这项工作的结果表明，小麦幼苗可以作为一种抗氧化剂，由于其自由基清除活性，可以建设性地控制或治疗许多健康并发症。从所有这些结果，我们相信小麦草可以用于人类的营养。
Precious contribution of plants in the field of medicine is very well known. Wheat (Triticum aestivum) seeds and seedlings are an important source of food and feed due to the presence of various health-promoting compounds. Proteomic analysis of three seed developmental stages (0, 8, and 16 days after germination [DAG]) of wheat was carried out using liquid chromatography-mass spectrometry. A total of 297 proteins were identified and their functional annotation revealed that a majority of them were involved in preventing many diseases, oxidative stress, primary metabolism, storage, and energy related mechanisms. Particularly to mention, peroxidases, superoxide dismutases, and cytochromes are abundantly present in wheatgrass. In the ferric-reducing antioxidant power assay, antioxidant activity was increased by 1.55 times after 16 DAG as compared to 0 DAG, however it was decreased after 8 DAG. The antioxidant activity of the plant extracts by DPPH had an increasing trend after all the three time points. The percent radical scavenging activity of extract by DPPH was 15, 22, and 30 after 0, 8, and 16 DAG, respectively. Observations obtained revealed that antioxidant power of the plants is directly proportional to the age of seedlings. Data attained on wheatgrass showing that it can be a strong antioxidant agent due to its free radical scavenging activity and could be used in stress and nourishing human health.Practical ApplicationWheatgrass contains minerals, phytochemicals, active enzymes, and vitamins that can be easily absorbed. The consumption of wheatgrass juice can give better health benefits. Information about beneficial properties of wheat grass juice is clearly mentioned in this work.Proteins found in wheatgrass are known to be involved in preventing many diseases, oxidative stress, primary metabolism, storage, and energy-related mechanisms. Results of this work revealed that Triticum aestivum seedlings can act as an antioxidant agent due to their free radical scavenging activity and can be constructive to control or treat many health complications. From all these results we believed that wheatgrass can be used for the nourishment of humans.