Domesticating Vigna Stipulacea: A Potential Legume Crop With Broad Resistance to Biotic Stresses
Domesticating Vigna Stipulacea: A Potential Legume Crop With Broad Resistance to Biotic Stresses
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DOI：
10.3389/fpls.2019.01607
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发表时间：
2019-12
影响因子：
5.6
通讯作者：
Yu Takahashi;H. Sakai;Yuki Yoshitsu;Chiaki Muto;T. Anai;M. Pandiyan;N. Senthil;N. Tomooka;K. Naito
中科院分区：
文献类型：
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作者：
Yu Takahashi;H. Sakai;Yuki Yoshitsu;Chiaki Muto;T. Anai;M. Pandiyan;N. Senthil;N. Tomooka;K. Naito
虽然将野生近缘种与现代栽培种杂交是引入胁迫耐受性等位基因的常用手段，但另一种选择是重新驯化已经耐受各种胁迫的野生物种。作为试验案例，我们选择了生长快、营养期短、抗病虫害能力强的豇豆。我们开发了一个甲基磺酸乙酯诱变群体，获得了三个种子休眠减少的突变体和一个豆荚脱落减少的突变体。我们将其中一个种子休眠性较低的突变体与野生型杂交，并证实其表型以孟德尔方式遗传。通过对紫背天葵基因组的从头组装和F2代的重测序，成功地鉴定出与种子休眠相关的单核苷酸多态性（SNP）。通过杂交和诱变突变体表型，我们将能够将黄花苜蓿变成一种原始的作物，但可以在没有杀虫剂的情况下种植。
Though crossing wild relatives to modern cultivars is a usual means to introduce alleles of stress tolerance, an alternative is de novo domesticating wild species that are already tolerant to various kinds of stresses. As a test case, we chose Vigna stipulacea Kuntze, which has fast growth, short vegetative stage, and broad resistance to pests and diseases. We developed an ethyl methanesulfonate–mutagenized population and obtained three mutants with reduced seed dormancy and one with reduced pod shattering. We crossed one of the mutants of less seed dormancy to the wild type and confirmed that the phenotype was inherited in a Mendelian manner. De novo assembly of V. stipulacea genome, and the following resequencing of the F2 progenies successfully identified a Single Nucleotide Polymorphism (SNP) associated with seed dormancy. By crossing and pyramiding the mutant phenotypes, we will be able to turn V. stipulacea into a crop which is yet primitive but can be cultivated without pesticides.