Genetically encoded alkenyl-pyrrolysine analogues for thiol-ene reaction mediated site-specific protein labeling
Genetically encoded alkenyl-pyrrolysine analogues for thiol-ene reaction mediated site-specific protein labeling
复制标题
用于硫醇烯反应介导的位点特异性蛋白质标记的基因编码的烯基吡咯赖氨酸类似物
DOI：
10.1039/c2sc20433a
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发表时间：
2012-01-01
期刊：
影响因子：
8.4
通讯作者：
Chen, Peng R.
中科院分区：
文献类型：
--
作者：
Li, Yiming;Yang, Maiyun;Chen, Peng R.
合成了一系列含烯烃的吡咯赖氨酸类似物，随后通过突变体 PylRS-tRNA 对以优异的效率在两个位点掺入蛋白质。该策略允许通过生物正交硫醇-烯连接反应对携带单或双基因编码烯烃柄的蛋白质进行位点特异性标记。
A series of alkene-bearing pyrrolysine analogues were synthesized and subsequently incorporated into proteins at two sites by a mutant PylRS-tRNA pair with excellent efficiency. This strategy allowed the site-specific labeling of proteins carrying single or double genetically encoded alkene handles via bioorthogonal thiol-ene ligation reactions.