Mapping of the mouse ornithine decarboxylase-related sequence family.
Mapping of the mouse ornithine decarboxylase-related sequence family.
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小鼠鸟氨酸脱羧酶相关序列家族的图谱。
DOI：
10.1007/bf00355431
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Elliott,RW
中科院分区：
文献类型：
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作者：
Richards-Smith,BA;Elliott,RW
已对与编码鸟氨酸脱羧酶（ODC）的mRNA同源的DNA序列家族进行了遗传分析，该家族包含小鼠基因组中的12个成员。用ODC cDNA探针对近交系小鼠DNA进行Southern杂交，检测了6个重组近交系小鼠品系的DNA限制性片段的遗传。这些变化之间的RI菌株的分布进行了比较与RI菌株分布模式（SDPs）以前映射的基因座。这使得能够鉴定9个独立的ODC相关基因座，其中8个可以定位于小鼠基因组的特定区域：染色体（Chr）1上的ODC-rs 1 near Lamb 2;染色体2上的ODC-rs 2 near Pspon;染色体6上的ODC序列的至少5-7个拷贝的复合基因座ODC-rs 5;染色体7近端上Abpa和Tam-1之间的ODC-rs6;染色体7远端上的ODC-rs7 near Ham-1;染色体8远端上的ODC-rs 12 near AgtandEmv-2; Odc-rs 8在Chr 12上与Igh复合物结合，Odc-rs 9在Chr 14上与Otf-3结合。因此，ODC相关序列家族包含一组基因组分散的“标记”基因座，并且可以在来自小鼠或细胞系的DNA中同时分析这些基因座中的几个的等位基因。从70个近交系小鼠的DNA已在所有九个ODC-RS位点的等位基因的特征。
A family of DNA sequences homologous to the mRNA encoding ornithine decarboxylase (ODC) and comprising ∼12 members in the mouse genome has been analyzed genetically. The inheritance of variant DNA restriction fragments detected by ODC cDNA probes on Southern blots of DNA from inbred strain mice was determined in six sets of recombinant inbred (RI) mouse strains. The distributions of these variations among the RI strains were then compared with the RI strain distribution patterns (SDPs) of previously mapped loci. This allowed the identification of nine independent ODC-related loci, of which eight could be localized to specific regions of the mouse genome:Odc-rs1nearLamb2on Chromosome (Chr) 1;Odc-rs2nearPspon Chr 2;Odc-rs5, a complex locus comprising at least 5–7 copies of the ODC sequence, associated withIgkon Chr 6;Odc-rs6betweenAbpaandTam-1on proximal Chr 7;Odc-rs7nearHbbon distal Chr 7;Odc-rs12nearAgtandEmv-2on distal Chr 8;Odc-rs8associated with theIghcomplex on Chr 12; andOdc-rs9nearOtf-3fon Chr 14. The ODC-related sequence family thus comprises a set of genomically dispersed “marker” loci, and alleles for several of these loci can be analyzed simultaneously in DNA from mice or cell lines. DNA from mice of 70 inbred strains has been characterized for alleles at all nineOdc-rsloci.