Gelatin-chondroitin-hyaluronan tri-copolymer scaffold for cartilage tissue engineering
Gelatin-chondroitin-hyaluronan tri-copolymer scaffold for cartilage tissue engineering
复制标题
DOI：
10.1016/s0142-9612(03)00383-1
复制
发表时间：
2003-11-01
期刊：
影响因子：
14
通讯作者：
Lin, FH
中科院分区：
文献类型：
--
作者：
Chang, CH;Liu, HC;Lin, FH
细胞合成和分泌细胞外基质（ECM）并进而受ECM调节的机制称为动态互惠性。本工作的目的是生产明胶/软骨素-6-硫酸盐/透明质酸三元共聚物，以模拟天然软骨基质，用作软骨组织工程的支架。所产生的支架具有约180 μ m的均匀孔径和75%的适当孔隙率。将猪软骨细胞接种到三共聚物支架上，并在培养皿或转瓶中培养2、3、4或5周。在转瓶培养中，软骨细胞均匀地分布在支架中，但在皮氏培养皿培养中较少。组织学检查可见ECM分泌。在转瓶培养中，软骨细胞保持其表型至少5周，如免疫组化所示，并合成II型胶原。这些结果表明明胶/硫酸软骨素/透明质酸三元共聚物具有用作软骨组织工程支架的潜力。(C)2003 Elsevier Ltd.保留所有权利。
The mechanism by which the cell synthesizes and secretes extracellular matrix (ECM) and is, in turn, regulated by the ECM is termed dynamic reciprocity. The aim of the present work was to produce a gelatin/chondoitin-6-sulfate/hyaluronan tri-copolymer to mimic natural cartilage matrix for use as a scaffold for cartilage tissue engineering. The scaffold produced had a uniform pore size of about 180 mum and adequate porosity of 75%. Porcine chondrocytes were seeded onto the tri-copolymer scaffold and cultured in Petri dishes or spinner flasks for 2, 3, 4, or 5 weeks. Chondrocytes were uniformly distributed in the scaffold in the spinner flask cultures, but less so in the Petri dish cultures. Secretion of ECM was found under histology examination. In spinner flask cultures, chondrocytes retained their phenotype for at least 5 weeks, as shown immumohistochemically, and synthesized type II collagen. These results show that gelatin/chondroitin sulfate/hyaluronan tri-copolymer has potential for use as a cartilage tissue engineering scaffold. (C) 2003 Elsevier Ltd. All rights reserved.