M‐07e cell bioassay detects stromal cell production of granulocyte‐macrophage colony stimulating factor and stem cell factor in normal and in diamond‐blackfan anemia bone marrow
M‐07e cell bioassay detects stromal cell production of granulocyte‐macrophage colony stimulating factor and stem cell factor in normal and in diamond‐blackfan anemia bone marrow
复制标题
M-07e 细胞生物测定检测正常和钻石-黑扇贫血骨髓中粒细胞-巨噬细胞集落刺激因子和干细胞因子的基质细胞产生
作者：
L. Bonsi;P. Strippoli;F. Bonifazi;Massimiliano Bonafeg;G. Bagnara;L. Vitale;M. Santucci;L. Pinto;P. Rosito;A. Pession;M. Brizzi;G. Avanzi;U. Ramenghi;V. Gabutti;G. Paolucci
Ten healthy donors and four patients with Diamond‐Blackfan anemia (DBA) have been investigated for granulocyte‐macrophage colony stimulating factor (GM‐CSF) and stem cell factor (SCF) production by bone marrow‐enriched fibroblasts (BMEF) in a highly sensitive biological assay on growth factor‐dependent M‐07e cells. M‐07e cells detected active soluble kit‐ligand from normal bone marrow fibroblasts as well as from DBA BMEF which produce constitutively significant amounts of SCF. Interleukin 1β (IL‐1β) induced a significant increase of soluble SCF from both normal and DBA BMEF. GM‐CSF was undetectable in unstimulated cultures, while its production by bone marrow microenvironmental cells was documented for both normal and DBA patients after IL‐1β stimulation in vitro.
影响因子：
20.3
作者：
J. Abkowitz;J. Abkowitz;K. Sabo;K. Sabo;B. Nakamoto;B. Nakamoto;C. Blau;C. Blau;Frank H. Martin-Frank-H.
通讯作者：
J. Abkowitz;J. Abkowitz;K. Sabo;K. Sabo;B. Nakamoto;B. Nakamoto;C. Blau;C. Blau;Frank H. Martin-Frank-H.
两名 Diamond-Blackfan 贫血患者的 c-kit 或其配体未出现异常。
影响因子：
20.3
作者：
Abkowitz,JL;Broudy,VC;Bennett,LG;Zsebo,KM;Martin,FH
通讯作者：
Martin,FH