A De novo Transcriptomic Approach to Identify Flavonoids and Anthocyanins "Switch-Off" in Olive (Olea europaea L.) Drupes at Different Stages of Maturation.
A De novo Transcriptomic Approach to Identify Flavonoids and Anthocyanins "Switch-Off" in Olive (Olea europaea L.) Drupes at Different Stages of Maturation.
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DOI：
10.3389/fpls.2015.01246
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发表时间：
2015
影响因子：
5.6
通讯作者：
Muzzalupo I
中科院分区：
文献类型：
--
作者：
Iaria DL;Chiappetta A;Muzzalupo I
对两个不同类型的橄榄核果进行了从头转录组的重建，监测了两个橄榄品种核果发育过程中的基因表达，分析了参与类黄酮和花青素途径的转录本在卡萨尼和白果中的表达，以及品种和发育阶段对橄榄果实基因表达的影响。在成熟过程中，橄榄树(Olea Europaea L.)的果实经历渐进性的色泽变化，特征是形成一个红棕色的“斑点”，逐渐延伸到中果皮的表皮和最里面部分。这一事件在白果品种中发现了一个例外，在该品种中，我们观察到叶绿素和其他色素的代谢之间的不稳定平衡，特别是花青素，其在成熟过程中的关闭促进了果实的白色。尽管橄榄树很重要，但关于它的基因组信息仍然缺乏。从开花后100天和130天采集的“白果”和“卡桑”橄榄核果建立了不同的RNA-seq文库，用于鉴定果实成熟过程中主要表型变化所涉及的转录本及其相应的表达模式。共获得103,359个转录本，在100-130个DAF过渡期间，分别有3792个和3064个转录本在“白果”和“卡萨尼斯”基因中差异表达。其中包括苯丙氨酸解氨酶(PAL)、肉桂酸4-羟基酶(C4H)、4-香豆酸-辅酶A连接酶(4CL)、查尔酮合成酶(CHS)、查尔酮异构酶(CHI)、黄烷酮3-羟基酶(F3H)、黄酮醇3‘-氢酶(F3’H)、黄酮醇合成酶(FLS)、二氢黄酮醇4-还原酶(DFR)、花青素合成酶(ANS)、UDP-葡萄糖：查尔酮异构酶(CHI)、黄酮醇3‘-羟基酶(F3’H)、黄酮醇合成酶(FLS)、二氢黄酮醇4-还原酶(DFR)、花青素合成酶(ANS)、查尔酮异构酶(CHI)、黄酮醇3‘-羟基酶(F3’H)、黄酮醇3‘5’-氢化氢酶(F3‘H)、黄酮醇4-还原酶(FLS)、花青素合成酶(ANS)。这些结果有助于缩小目前关于新陈代谢过程的信息差距，包括与橄榄果实色素沉着有关的信息。
A de novo transcriptome reconstruction of olive drupes was performed in two genotypes Gene expression was monitored during drupe development in two olive cultivars Transcripts involved in flavonoid and anthocyanin pathways were analyzed in Cassanese and Leucocarpa cultivars Both cultivar and developmental stage impact gene expression in Olea europaea fruits. Highlights During ripening, the fruits of the olive tree (Olea europaea L.) undergo a progressive chromatic change characterized by the formation of a red-brown “spot” which gradually extends on the epidermis and in the innermost part of the mesocarp. This event finds an exception in the Leucocarpa cultivar, in which we observe a destabilized equilibrium between the metabolisms of chlorophyll and other pigments, particularly the anthocyanins whose switch-off during maturation promotes the white coloration of fruits. Despite its importance, genomic information on the olive tree is still lacking. Different RNA-seq libraries were generated from drupes of “Leucocarpa” and “Cassanese” olive genotypes, sampled at 100 and 130 days after flowering (DAF), and were used in order to identify transcripts involved in the main phenotypic changes of fruits during maturation and their corresponding expression patterns. A total of 103,359 transcripts were obtained and 3792 and 3064 were differentially expressed in “Leucocarpa” and “Cassanese” genotypes, respectively, during 100–130 DAF transition. Among them flavonoid and anthocyanin related transcripts such as phenylalanine ammonia lyase (PAL), cinnamate 4-hydroxylase (C4H), 4-coumarate-CoA ligase (4CL), chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavonol 3′-hydrogenase (F3′H), flavonol 3′5 ′-hydrogenase (F3′5′H), flavonol synthase (FLS), dihydroflavonol 4-reductase (DFR), anthocyanidin synthase (ANS), UDP-glucose:anthocianidin: flavonoid glucosyltransferase (UFGT) were identified. These results contribute to reducing the current gap in information regarding metabolic processes, including those linked to fruit pigmentation in the olive.