A Role for the Anti-Viral Host Defense Mechanism in the Phylogenetic Divergence in Baculovirus Evolution.
A Role for the Anti-Viral Host Defense Mechanism in the Phylogenetic Divergence in Baculovirus Evolution.
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DOI：
10.1371/journal.pone.0156394
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Sako Y
中科院分区：
文献类型：
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作者：
Nagamine T;Sako Y
虽然系统发育分析经常表明病毒和宿主生物之间存在共同进化关系，但很少有在微观进化水平上报道的例子。在这里，我们展示了一个可能的例子，其中物种特异性抗病毒反应可能驱动昆虫病毒进化的系统发育差异。两种杆状病毒，加利福尼亚自签名多核多角体病毒（AcMNPV）和家蚕多核多角体病毒（BmNPV）具有高度的DNA序列相似性，但表现出不重叠的宿主特异性。在我们对其宿主范围测定的研究中，我们发现AcMNPV-P143 （AcP143）可以阻止BmNPV在家蚕b细胞中的复制，而bmmnpv -P143 （BmP143）或来自宿主范围扩展表型的P143杂交蛋白则不能。这表明，家蚕B. mori细胞对AcMNPV的抗性依赖于AcP143的识别，而BmNPV使用BmP143来逃避这种识别。基于这些数据，我们提出了一种昆虫-杆状病毒共同进化的情景，其中家蚕的祖先利用了acmnpv抗性机制；AcMNPV通过P143突变抵消了这种抗性，导致BmNPV的诞生。
Although phylogenic analysis often suggests co-evolutionary relationships between viruses and host organisms, few examples have been reported at the microevolutionary level. Here, we show a possible example in which a species-specific anti-viral response may drive phylogenic divergence in insect virus evolution. Two baculoviruses, Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Bombyx mori nucleopolyhedrovirus (BmNPV), have a high degree of DNA sequence similarity, but exhibit non-overlapping host specificity. In our study of their host-range determination, we found that BmNPV replication in B. mori cells was prevented by AcMNPV-P143 (AcP143), but not BmNPV-P143 (BmP143) or a hybrid P143 protein from a host-range expanded phenotype. This suggests that AcMNPV resistance in B. mori cells depends on AcP143 recognition and that BmNPV uses BmP143 to escapes this recognition. Based on these data, we propose an insect-baculovirus co-evolution scenario in which an ancestor of silkworms exploited an AcMNPV-resistant mechanism; AcMNPV counteracted this resistance via P143 mutations, resulting in the birth of BmNPV.