Fluorescent nucleosides with 'on-off' switching function, pH-responsive fluorescent uridine derivatives.
Fluorescent nucleosides with 'on-off' switching function, pH-responsive fluorescent uridine derivatives.
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DOI：
10.1016/j.bmcl.2012.02.092
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发表时间：
2012-04
影响因子：
2.7
通讯作者：
Yoshio Saito;S. Miyamoto;Azusa Suzuki;Katsuhiko Matsumoto;T. Ishihara;I. Saito
中科院分区：
文献类型：
--
作者：
Yoshio Saito;S. Miyamoto;Azusa Suzuki;Katsuhiko Matsumoto;T. Ishihara;I. Saito
我们合成了各种pH响应性荧光脱氧尿苷衍生物（1a-g）。这些核苷在酸性至中性的pH值下，在470- 600 nm处显示出独特的荧光。特别地，1f仅在pH为3.1-7.2的范围内表现出强荧光，pKa为6.1。这种pH敏感的荧光核苷可以用作“开-关”荧光开关，用于监测生物系统中的pH变化，特别是用于癌细胞检测。
We synthesized various pH-responsive fluorescent deoxyuridine derivatives (1a–g). These fluorescent nucleosides exhibited distinctive fluorescence at 470–600nm in aqueous solvents containing methanol only at acidic to neutral pH values. In particular, 1f exhibited strong fluorescence only at pH range of 3.1–7.2 with a pKaof 6.1. Such pH-sensitive fluorescent nucleosides can be used as ‘on–off’ fluorescence switch for monitoring pH change in biological systems, particularly for cancer cell detection.