Transcriptional landscape of alternative splicing during peripheral nerve injury
Transcriptional landscape of alternative splicing during peripheral nerve injury
复制标题
周围神经损伤期间选择性剪接的转录景观
DOI：
10.1002/jcp.27446
复制
发表时间：
2019-05-01
影响因子：
5.6
通讯作者：
Yi, Sheng
中科院分区：
文献类型：
--
作者：
Zhao, Lili;Yi, Sheng
选择性剪接（AS）在包括神经系统在内的许多组织和器官中调节各种生物活性。然而，周围神经修复和再生过程中AS事件的存在和具体作用仍然在很大程度上不确定。在目前的研究中，通过绘制剪接交叉序列读段，我们在坐骨神经挤压后的大鼠坐骨神经残端中鉴定了AS事件和相关剪接基因。将神经夹伤后1、4、7和14天的AS相关基因与0天的AS相关基因进行比较，以发现坐骨神经夹伤诱导的选择性剪接基因。然后将这些损伤诱导的可变剪接基因分类为疾病和生物学功能，遗传网络和经典信号通路。生物信息学分析表明，这些可变剪接基因主要与免疫应答、细胞生长和细胞功能维持相关。我们的研究阐明了周围神经损伤后的AS事件，可能有助于加深我们对周围神经再生的分子机制的理解。
Alternative splicing (AS) regulates a variety of biological activities in numerous tissues and organs, including the nervous system. However, the existence and specific roles of AS events during peripheral nerve repair and regeneration remain largely undetermined. In the current study, by mapping splice‐crossing sequence reads, we identified AS events and relevant spliced genes in rat sciatic nerve stumps following sciatic nerve crush. AS‐related genes at 1, 4, 7, and 14 days post nerve crush were compared with those at 0 day to discover alternatively spliced genes induced by sciatic nerve crush. These injury‐induced alternatively spliced genes were then categorized to diseases and biological functions, genetic networks, and canonical signaling pathways. Bioinformatic analysis indicated that these alternatively spliced genes were mainly correlated to immune response, cellular growth, and cellular function maintenance. Our study elucidated AS events following peripheral nerve injury and might help deepen our understanding of the molecular mechanisms underlying peripheral nerve regeneration.