Direct Current Helium Plasma for In vivo Delivery of Plasmid DNA Encoding Erythropoietin to Murine Skin.
Direct Current Helium Plasma for In vivo Delivery of Plasmid DNA Encoding Erythropoietin to Murine Skin.
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DOI：
10.1615/plasmamed.2017019506
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发表时间：
2017-01-01
期刊：
影响因子：
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通讯作者：
Connolly, Richard J
中科院分区：
文献类型：
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作者：
Jaroszeski, Mark J;Harvey-Chapman, Taryn;Connolly, Richard J
在体内使用电场传递DNA，称为电穿孔，有可能广泛影响疫苗接种和疾病治疗。这方面的证据来自大量最近完成和正在进行的临床试验。用于向组织施加电场的方法传统上涉及金属电极与组织之间的接触。在这项研究中，我们研究了使用氦等离子体作为一种非接触的方法，用于电治疗组织的方式，导致在小鼠皮肤中的外源DNA的摄取和表达。更具体地说，我们的目标是证明编码模型分泌蛋白的DNA可以在血液中被递送、检测，并保持产生其已知生物学效应的功能。小鼠促红细胞生成素（EPO）是模型分泌蛋白。结果清楚地表明，皮内DNA注射，然后血浆处理2分钟，导致血液中EPO水平升高，相应的血红蛋白增加，相对于单独的DNA注射具有统计学显著性。
The use of electric fields in vivo to deliver DNA, called electroporation, has the potential to broadly impact vaccination and disease treatment. The evidence for this has emerged from a large number of recently completed and ongoing clinical trials. The methods for applying electric fields to tissues traditionally involve contact between metal electrodes and the tissue. In this study, we investigated the use of helium plasma as a noncontact method for electrically treating tissue in a manner that results in the uptake and expression of foreign DNA in murine skin. More specifically, our goal was to demonstrate that DNA encoding a model-secreted protein could be delivered, detected in the blood, and remain functional to produce its known biological effect. Murine erythropoietin (EPO) was the model-secreted protein. Results clearly demonstrated that an intradermal DNA injection followed by plasma treatment for 2 min resulted in elevated levels of EPO in the blood and corresponding hemoglobin increases that were statistically significant relative to DNA injection alone.