Effects of self-assembling peptide hydrogel scaffold on bone regeneration with recombinant human bone morphogenetic protein-2.
Effects of self-assembling peptide hydrogel scaffold on bone regeneration with recombinant human bone morphogenetic protein-2.
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DOI：
10.11607/jomi.te09
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发表时间：
2013-09
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影响因子：
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通讯作者：
M. Ikeno;H. Hibi;K. Kinoshita;H. Hattori;M. Ueda
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作者：
M. Ikeno;H. Hibi;K. Kinoshita;H. Hattori;M. Ueda
目的研究重组人骨形态发生蛋白-2(rhBMP-2)自组装多肽水凝胶支架在兔颅骨模型中的成骨作用。材料与方法选用成年新西兰大白兔5只。每个人都接受了四个钛圆柱体，这些圆柱体被放入在头盖骨外侧皮质骨上形成的穿孔狭缝中。圆柱体填充以下测试材料：(1)空白对照；(2)rhBMP-2；(3)PuraMatrix(PM)，一种由16个氨基酸序列组成的具有三维结构的合成自组装肽(RADA16-I)；和(4)PM/rhBMP-2。每个圆柱体上都覆盖着一个钛盖子。8周后处死动物，取组织切片进行组织形态计量学分析。结果组织形态计量学分析显示，PM/rhBMP-2组再生组织明显多于空白对照组。再生组织平均面积分别为35.80%±10.35%(对照组)、47.94%±5.65%(rhBMP-2)、48.94%±11.33%(PM)和58.06%±14.84%(PM/rhBMP-2)。新生骨的平均面积为9.39%±4.34%(对照组)、14.03%±2.25%(rhBMP-2)、13.99%±2.15%(PM)和16.61%±3.79%(PM/rhBMP-2)。与空白对照圆柱体相比，单独使用rhBMP-2和PM都不能显著促进骨再生。结论复合重组人骨形态发生蛋白-2的PM能显著促进兔增骨模型的骨再生。PM有望成为一种有用的替代合成材料，作为rhBMP-2的载体用于骨再生。
PURPOSE The objective of this pilot study was to histologically evaluate bone regeneration using a self-assembling peptide hydrogel scaffold with recombinant human bone morphogenetic protein-2 (rhBMP-2) in a rabbit calvaria model. MATERIALS AND METHODS Five adult New Zealand White rabbits were used for the study. Each received four titanium cylinders, which were placed into perforated slits made in the outer cortical bone of the calvaria. The cylinders were filled with the following test materials: (1) unfilled control; (2) rhBMP-2; (3) PuraMatrix (PM), a synthetic self-assembling peptide (RADA16-I) consisting of a 16-amino acid sequence and with a three-dimensional structure; and (4) PM/rhBMP-2. Each cylinder was covered with a titanium lid. After 8 weeks, the animals were sacrificed, and ground sections were obtained for histomorphometric analysis. RESULTS Histomorphometric analysis showed that regenerated tissue in the cylinder with PM/rhBMP-2 was significantly increased compared to the empty control. The mean area values of regenerated tissue in the cylinders were 35.80% ± 10.35% (control), 47.94% ± 5.65% (rhBMP-2), 48.94% ± 11.33% (PM), and 58.06% ± 14.84% (PM/rhBMP-2). The mean area values of newly formed bone in the cylinders were 9.39% ± 4.34% (control), 14.03% ± 2.25% (rhBMP-2), 13.99% ± 2.15% (PM), and 16.61% ± 3.79% (PM/rhBMP-2). Neither rhBMP-2 nor PM alone significantly enhanced bone regeneration compared to the empty control cylinder. CONCLUSIONS PM with rhBMP-2 significantly enhanced bone regeneration on the bone augmentation model in a rabbit. PM promises to be a useful alternative synthetic material as a carrier for rhBMP-2 for bone regeneration.