A bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors
A bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors
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DOI：
10.1038/nbt1120
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发表时间：
2005-08-01
影响因子：
46.9
通讯作者：
Wolfe, SA
中科院分区：
文献类型：
--
作者：
Meng, XD;Brodsky, MH;Wolfe, SA
转录因子的dna结合特异性可用于计算预测调节基因表达的顺式调控模块（CRMs）(1)。然而，缺乏大多数转录因子的特异性数据限制了这种方法的广泛实施。我们开发了一种细菌单杂交系统，提供了一种简单快速的方法来确定转录因子的dna结合特异性。利用这项技术，我们成功地确定了7个先前表征的转录因子和一个新的转录因子，果蝇黑胃因子odd -skip的dna结合特异性。利用这些信息成功预测了odd -skip的调控靶点，证明了细菌单杂交系统产生的数据与体内功能相关。
The DNA-binding specificities of transcription factors can be used to computationally predict cis-regulatory modules (CRMs) that regulate gene expression(1). However, the absence of specificity data for the majority of transcription factors limits the widespread implementation of this approach. We have developed a bacterial one-hybrid system that provides a simple and rapid method to determine the DNA-binding specificity of a transcription factor. Using this technology, we successfully determined the DNA-binding specificity of seven previously characterized transcription factors and one novel transcription factor, the Drosophila melanogaster factor Odd-skipped. Regulatory targets of Odd-skipped were successfully predicted using this information, demonstrating that the data produced by the bacterial one-hybrid system are relevant to in vivo function.