HOPE-BAL: Improved Molecular Diagnostics by Application of a Novel Technique for Fixation and Paraffin Embedding
HOPE-BAL: Improved Molecular Diagnostics by Application of a Novel Technique for Fixation and Paraffin Embedding
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DOI：
10.1369/0022155411404417
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发表时间：
2011-06-01
影响因子：
3.2
通讯作者：
Goldmann, Torsten
中科院分区：
文献类型：
--
作者：
Marwitz, Sebastian;Abdullah, Mahdi;Goldmann, Torsten
支气管肺泡灌洗（BAL）及其细胞已被广泛用于临床诊断和研究问题的多功能工具，多年来。由于没有足够的长期储存的可能性，作者在这项研究中探索了一种新的固定剂的实用性，用于固定和石蜡包埋人灌洗液细胞的可能性，实施标准的分子生物学技术。对不同肺部疾病（哮喘、慢性阻塞性肺疾病、结核、结节病、肺气肿和纤维化）患者的HOPE固定、石蜡包埋的BAL细胞进行免疫组织化学、原位杂交、定量聚合酶链反应和转录微阵列分析。此外，进行二维凝胶电泳以评估用于研究、诊断和进一步在生物库中实施的可能应用范围。作者通过靶向一些示例性分子，展示了筛选和验证HOPE-BAL用于新生物标志物的能力。转化生长因子β信号通路可能在感染和哮喘的免疫调节中发挥重要作用。此外，触珠蛋白在哮喘和结节病中过表达。由于核酸，蛋白质和形态结构的良好保存，HOPE-BAL是肺部医学增强分子诊断和生物库的一步。（J Histochem Cytochem 59：601-614，2011）
The bronchoalveolar lavage (BAL) and its cells have been widely used as a support for clinical diagnosis and as a versatile tool for research questions since many years. Because there are no sufficient possibilities of long-term storage, the authors explore in this study the utility of a new fixative for fixation and paraffin embedding of human lavage cells with the possibility of implementing standard molecular biology techniques. HOPE-fixed, paraffin-embedded BAL cells of patients with different lung diseases (asthma, chronic obstructive pulmonary diseases, tuberculosis, sarcoidosis, emphysema, and fibrosis) were subjected to immunohistochemistry, in situ hybridization, quantitative polymerase chain reaction, and transcription microarray analysis. Furthermore, two-dimensional gel electrophoresis was conducted to evaluate the range of possible applications for research, diagnostics, and further implementing in biobanks. The authors show, by targeting some exemplary molecules, the power of screening and validating HOPE-BAL for new biomarkers. The transforming growth factor beta signaling pathway may play a central role in immunomodulation upon infection as well as asthma. Furthermore, haptoglobin was overexpressed in asthma and sarcoidosis. Because of the excellent preservation of nucleic acids, protein, and morphologic structures, HOPE-BAL is a step forward into enhanced molecular diagnostics and biobanking in pulmonary medicine. (J Histochem Cytochem 59:601-614, 2011)