Serum immunoglobulin G immune response to Helicobacter pylori antigens in mongolian gerbils
Serum immunoglobulin G immune response to Helicobacter pylori antigens in mongolian gerbils
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DOI：
10.1128/jcm.39.4.1283-1288.2001
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发表时间：
2001-04-01
影响因子：
9.4
通讯作者：
Ota, H
中科院分区：
文献类型：
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作者：
Kumagai, T;Yan, J;Ota, H
蒙古沙鼠幽门螺杆菌感染模型是一种模拟人类疾病的动物模型。我们检测了对Ii的血清免疫应答。pylori感染沙鼠的酶联免疫吸附试验（ELISA）和蛋白质印迹法，都与全细胞（H。pylori）提取物。pylori菌株ATCC 43504。H后1、2、4、8、12、26、38和52周收集血清。幽门螺杆菌接种。以69只正常沙鼠及其血清为对照，ELISA的特异性为95.7%。血清阳性频率随着时间的推移而增加：接种沙鼠的血清样本中有2/10（20%）、7/10（70%）和7/7（100%）呈H阳性。pylori感染。免疫印迹分析表明，主要的免疫球蛋白G（IgG）对低分子量（25-，30-，和20-kDa）蛋白质的反应后出现的滞后期2至8周后接种。接种后12周，观察到针对160-、150-、110-、120-、80-、66-和63-kDa蛋白的抗体。通过N-末端氨基酸测序，早期反应性30-kDa蛋白被鉴定为尿素酶α亚基。26周后，可以区分两组动物：胃溃疡组血清抗Hpylori IgG水平显著高于增生组（P <0.05），而增生组血清抗Hpylori IgG水平显著低于溃疡组（P <0.05（P = 0.001）。此外，在Western印迹分析中，溃疡组中产生针对26、25和20 kDa幽门螺杆菌蛋白的抗体的动物比例高于增生性息肉组（75 - 100%对17 - 50%）。这些结果强调了宿主免疫应答在幽门螺杆菌相关胃病变发展中的重要性。
The Mongolian gerbil model For Helicobacter pylori infection is an animal model that mimics human disease. We examined the serum immune response to Ii. pylori infection in gerbils by enzyme-linked immunosorbent assay (ELISA) and Western blotting, both with whole-cell (H. pylori) extracts, A total of 66 7-week-old specific-wpathogen-free male gerbils were inoculated orogastrically with H. pylori strain ATCC 43504. Sera were collected 1, 2, 4, 8, 12, 26, 38, and 52 weeks after H. pylori inoculation. Sixty-nine noninfected gerbils and their sera were used as controls, The specificity of the ELISA was 95.7%. The frequency of seropositivity increased over time: 2 of 10 (20%), 7 of 10 (70%), and 7 of 7 (100%) samples of sera from inoculated gerbils were positive for H. pylori at 2, 4, and 8 weeks postinoculation, respectively. Western blot assays showed that the primary immunoglobulin G (IgG) response against low-molecular-mass (25-, 30-, and 20-kDa) proteins appeared after a lag period of 2 to 8 weeks after inoculation. Antibodies against 160-, 150-, 110-, 120-, 80-, 66-, and 63-kDa proteins were observed 12 weeks after inoculation, The early reactive 30-kDa protein was identified as a urease oc subunit by N-terminal amino acid sequencing, After 26 weeks, two groups of animals could be distinguished: one group developed ulcers (n = 5), and the other developed hyperplastic polyps without ulcers (n = 19), Gerbils in the gastric ulcer group showed significantly higher serum anti-H pylori IgG levels than did gerbils in the hyperplastic group (P = 0.001) as measured by ELISA, Furthermore, a higher proportion of animals developed antibodies to H pylori proteins of 26, 25, and 20 kDa in the ulcer group than those animals with hyperplastic polyps (75 to 100% versus 17 to 50%) in Western blot assays. These results highlight the importance of the immune response of the host in the development of Ii pylori-related gastric lesions.