Owl monkey gene mapping: the assignment of gene loci for catalase, beta-globin gene cluster, HRAS1, insulin, and parathyroid hormone.
Owl monkey gene mapping: the assignment of gene loci for catalase, beta-globin gene cluster, HRAS1, insulin, and parathyroid hormone.
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猫头鹰猴基因定位：过氧化氢酶、β-珠蛋白基因簇、HRAS1、胰岛素和甲状旁腺激素的基因位点分配。
DOI：
10.1159/000132298
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Bruns,G
中科院分区：
文献类型：
--
作者：
Ma,NS;Gerhard,DS;Housman,DE;Orkin,S;Bruns,G
利用体细胞遗传学和Southern blot杂交技术，我们绘制了猫头鹰猴的5个结构基因图谱，分别编码过氧化氢酶（CAT）、β-珠蛋白基因簇（HBBC）、c-Ha-ras1 （HRAS1）、胰岛素（INS）和甲状旁腺激素（PTH）。这5个位点均定位于猫头鹰猴VI核型（2n = 49,50）的19号染色体上；CAT、HBBC、INS和pth4可以分配到核型V的4号染色体上（2n = 46），而ecat和HBBC可以分配到核型III的2号染色体上（2n = 53）。利用原位杂交技术，将catgene精确定位在染色体2q端粒末端的中间区域和β-珠蛋白基因簇上（K-ΠI）。我们的结果为这些基因位点的进化史提供了重要的见解。虽然这些基因座在啮齿类动物（如小鼠）中至少分为两个主要部分，但我们的研究结果表明，在高等灵长类动物中，单个染色体臂是守恒的。
Using somatic cell genetics and Southern blot hybridization, we have mapped five structural genes in the owl monkey, coding for catalase (CAT), the β-globin gene cluster (HBBC),c-Ha-ras1 (HRAS1), insulin (INS), and parathyroid hormone (PTH). All five loci are mapped to chromosome 19 of karyotype VI (2n = 49,50) of the owl monkey;CAT,HBBC,INS,andPTHcan be assigned to chromosome 4 of karyotype V (2n = 46), whileCATand HBBC can be assigned to chromosome 2 of karyotype III (2n = 53). Using in situ hybridization, theCATgene was precisely mapped on the mid-region and the β-globin gene cluster on the telomeric end of chromosome 2q(K-ΠI). Our results provide significant insight into the evolutionary history of these gene loci. While these loci are separated into at least two major segments in rodents such as the mouse, our results suggest conservation of a single chromosome arm among higher primates.