Localization, quantitation, and in situ detection of specific peptide MHC class I complexes using a monoclonal antibody
Localization, quantitation, and in situ detection of specific peptide MHC class I complexes using a monoclonal antibody
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DOI：
10.1016/s1074-7613(00)80447-1
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发表时间：
1997-06-01
期刊：
影响因子：
32.4
通讯作者：
Germain, RN
中科院分区：
文献类型：
--
作者：
Porgador, A;Yewdell, JW;Germain, RN
CD 8（+）T淋巴细胞识别与MHC I类分子结合的短肽抗原。现有的方法不能确定这些配体在单个细胞上的数量和分布，也不能检测组织中的抗原呈递细胞。在这里，我们描述了一种方法，用于引发和识别单克隆抗体特异性的特定肽-MHC I类组合。一种这样的抗体可以识别抗原复合物，其检测极限接近T细胞的检测极限。我们使用这种抗体来确定病毒感染后产生的肽-I类复合物的数量，以确定细胞混合物中的抗原呈递细胞，以确定细胞内肽-MHC I类相互作用的位点，并在体内感染后可视化携带特异性肽-MHC I类复合物的细胞。类似的抗体可证明可用于癌症、感染性疾病和自身免疫性疾病的诊断或治疗目的。
CD8(+) T lymphocytes recognize antigens as short peptides bound to MHC class I molecules. Available methods cannot determine the number and distribution of these ligands on individual cells or detect antigen-presenting cells in tissues. Here we describe a method for eliciting and identifying monoclonal antibodies specific for a particular peptide-MHC class I combination. One such antibody can identify antigen complexes with a limit of detection approaching that of T cells. We used this antibody to determine the number of peptide-class I complexes generated upon viral infection, to identify antigen-presenting cells in cell mixtures, to determine the site of peptide-MHC class I interaction inside cells, and to visualize cells bearing specific peptide-MHC class I complexes after in vivo infection. Similar antibodies may prove useful for diagnostic or therapeutic purposes in cancer, infectious diseases, and autoimmune disorders.