Keratinocyte stem cells, label-retaining cells and possible genome protection mechanisms
Keratinocyte stem cells, label-retaining cells and possible genome protection mechanisms
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DOI：
10.1111/j.1087-0024.2004.09305.x
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发表时间：
2004-09-01
影响因子：
--
通讯作者：
Potten, CS
中科院分区：
文献类型：
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作者：
Potten, CS
干细胞是整个组织所依赖的关键细胞。在这里，我们定义和讨论什么是角质形成细胞干细胞和已知的。研究这些细胞的一种方法是通过它们长时间保留放射性标记的胸苷的能力（标记保留细胞，LRC）。过去认为其潜在机制是缓慢循环，但更可能的解释是新旧DNA链的选择性分离（凯恩斯假说）。在小肠中的实验表明，这里的干细胞选择性地分选它们的DNA并成为LRC。p53在干细胞生物学中的可能作用。
Stem cells are the crucial cells upon which the entire tissue is dependent. Here we define and discuss what is meant by and known about keratinocyte stem cells. One way in which these cells have been studied is by their ability to retain radioactivity labelled thymidine for long periods of time (label retaining cells, LRCs). The underlying mechanism has been assumed in the past to be slow cycling but a more likely explanation is the selective segregation of old and new DNA strands (Cairns's hypothesis). Experiments in the small intestine indicate that the stem cells here are selectively sorting their DNA and becoming LRCs. A possible role for p53 in stem cell biology is presented.