INORGANIC ARSENIC EFFECTS ON HUMAN LYMPHOCYTE STIMULATION AND PROLIFERATION
INORGANIC ARSENIC EFFECTS ON HUMAN LYMPHOCYTE STIMULATION AND PROLIFERATION
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DOI：
10.1016/0165-7992(92)90139-9
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发表时间：
1992-10-01
期刊：
影响因子：
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通讯作者：
OSTROSKYWEGMAN, P
中科院分区：
文献类型：
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作者：
GONSEBATT, ME;VEGA, L;OSTROSKYWEGMAN, P
淋巴细胞培养暴露于高水平的砷中毒的个人表现出较慢的细胞周期动力学比文化低暴露的个人。由于这种增殖的差异可能是由于慢性砷暴露，无机砷在人全血淋巴细胞培养的体外效应进行了研究。当淋巴细胞在收获前的最后24小时内暴露于与暴露受试者血液中发现的浓度相似的亚砷酸盐和砷酸盐（10（-7）、10（-8）和10（-9）M）时，观察到剂量相关的增殖抑制。在培养开始时，在存在或不存在植物血凝素（PHA）的情况下，用10（-9）M亚砷酸盐和砷酸盐处理培养物2、6和24 h。刺激和增殖的抑制与治疗时间的长短直接相关。结果表明，在测试的浓度下，砷和砷酸盐损害淋巴细胞的刺激和增殖，并确认事实，慢性砷暴露可以影响全血淋巴细胞的增殖。
Lymphocyte cultures from individuals exposed to high levels of hydroarsenicism showed a slower cell cycle kinetics than cultures from low-exposed individuals. Since this difference in proliferation could be due to chronic arsenic exposure, the in vitro effects of inorganic arsenic in human whole blood lymphocyte cultures were investigated. When lymphocytes were exposed to concentrations of arsenite and arsenate similar to those found in the blood of exposed subjects (10(-7), 10(-8) and 10(-9) M) during the last 24 h before harvesting, a dose-related inhibition of proliferation was observed. Cultures were also treated with 10(-9) M of arsenite and arsenate for 2, 6 and 24 h at the beginning of the cultures in the presence or absence of phytohemagglutinin (PHA). Inhibition of stimulation and proliferation was directly related to the length of treatment. The results show that, at the concentrations tested, arsenite and arsenate impair lymphocyte stimulation and proliferation and confirm the fact that chronic arsenic exposure can affect the proliferation of whole blood lymphocytes.