Moderate abiotic stresses increase rhizome growth and outgrowth of axillary buds in Alstroemeria cultured in vitro
Moderate abiotic stresses increase rhizome growth and outgrowth of axillary buds in Alstroemeria cultured in vitro
复制标题
中等非生物胁迫促进体外培养的六出花根茎生长和腋芽生长
DOI：
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发表时间：
2012
影响因子：
3
通讯作者：
G. Klerk
中科院分区：
文献类型：
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作者：
P. Pumisutapon;R. Visser;G. Klerk
通过根茎外植体的侧根的强制长出，在体外增殖Alstroemeria。由于顶端优势强，根茎生长差，繁殖率很低。我们在这里报告，适度的非生物胁迫刺激根茎生长和侧根的生长，并相应地增加繁殖。根茎外植体暴露于热通过热水处理（HWT）或通过热空气处理。当在30-40 °C范围内施用1或2小时时，两者都增加根茎生长。最大强化75%。其他非生物胁迫也进行了研究。低温（0 °C）和部分厌氧显著促进根茎生长。干旱和盐渍化引起的增加在统计上不显著。由于地下储藏组织如根茎是适应生存的气候压力，我们推测，增加水槽强度的根茎诱导适度的压力适应。中度热胁迫（38 °C HWT，1小时）也导致在中度胁迫后几小时保护Alstroemeria小植株免受严重热胁迫（45 °C HWT，1-2小时）。所有非生物胁迫也增加了侧根的生长。
Alstroemeria is multiplied in vitro by forced outgrowth of lateral rhizomes from rhizome explants. The multiplication rate is very low because of strong apical dominance and poor rhizome growth. We report here that moderate abiotic stresses stimulate both rhizome growth and outgrowth of lateral rhizomes, and accordingly increase multiplication. Rhizome explants were exposed to heat by a hot-water treatment (HWT) or by a hot-air treatment. Both increased rhizome growth when applied for 1 or 2 h in the range of 30–40 °C. The maximal enhancement was 75 %. Other abiotic stresses were also examined. Cold (0 °C) and partial anaerobiosis increased rhizome growth significantly. The increases brought about by drought and salinity were not statistically significant. Because underground storage tissues like rhizomes are adaptations to survive climatic stresses, we presume that the increased sink-strength of rhizomes induced by moderate stress is related to stress adaptation. Moderate heat stress (38 °C HWT, 1 h) also resulted in protection of Alstroemeria plantlets from severe heat stress (45 °C HWT, 1–2 h) a few hours after the moderate stress. All abiotic stresses also increased the outgrowth of lateral rhizomes.