Recent progress in voltage-sensitive dye imaging for neuroscience.
Recent progress in voltage-sensitive dye imaging for neuroscience.
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DOI：
10.1166/jnn.2014.9531
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发表时间：
2014-07
影响因子：
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通讯作者：
V. Tsytsarev;Lun-De Liao;K. Kong;Yu-Hang Liu;R. Erzurumlu;M. Olivo;N. Thakor
中科院分区：
文献类型：
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作者：
V. Tsytsarev;Lun-De Liao;K. Kong;Yu-Hang Liu;R. Erzurumlu;M. Olivo;N. Thakor
电压敏感染料成像（VSDi）能够以合理的空间和时间分辨率可视化大脑不同区域的信息处理。VSDi采用不同的化学化合物将神经活动直接转化为内在光信号的变化。物理上，电压敏感染料（VSD）是驻留在神经膜中的化学探针，并响应于膜电位的变化而改变其荧光或吸光度。基于这些特征，VSD可以分为两组-吸收和荧光。VSD i的空间和时间分辨率主要受光学成像设置的技术特性（例如，计算机和光敏器件-电荷耦合器件（CCD）照相机或光电二极管阵列）。本文就VSD的发展、VSD成像技术及其在脑功能成像中的应用作一简要综述。
Voltage-sensitive dye imaging (VSDi) enables visualization of information processing in different areas of the brain with reasonable spatial and temporal resolution. VSDi employs different chemical compounds to transduce neural activity directly into the changes in intrinsic optical signal. Physically, voltage-sensitive dyes (VSDs) are chemical probes that reside in the neural membrane and change their fluorescence or absorbance in response to membrane potential changes. Based on these features, VSDs can be divided into two groups-absorbance and fluorescence. The spatial and temporal resolution of the VSDi is limited mainly by the technical characteristics of the optical imaging setup (e.g., computer and light-sensitive device-charge-coupled device (CCD) camera or photodiode array). In this article, we briefly review the development of the VSD, technique of VSDi and applications in functional brain imaging.