Rapid telomere movement in meiotic prophase is promoted by NDJ1, MPS3, and CSM4 and is modulated by recombination
Rapid telomere movement in meiotic prophase is promoted by NDJ1, MPS3, and CSM4 and is modulated by recombination
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DOI：
10.1016/j.cell.2008.04.047
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发表时间：
2008-06-27
期刊：
影响因子：
64.5
通讯作者：
Dresser, Michael E.
中科院分区：
文献类型：
--
作者：
Conrad, Michael N.;Lee, Chih-Ying;Dresser, Michael E.
减数分裂中基因组的单倍体化需要染色体被排他地分选成由联会复合体（SC）和交换稳定的对。这种分类和配对伴随着减数分裂前期活跃的染色体定位，其中端粒聚集在纺锤体极点附近形成花束，然后分散在核膜周围。我们现在描述端粒主导的快速前期运动（RPM），经常超过1亩/秒，并坚持整个减数分裂前期。花束的形成和RPM依赖于NDJ 1，MPS 3，和这个途径的新成员，CSM 4，它编码一个减数分裂特异性核膜蛋白，特别是端粒移动所需的。RPM启动独立的重组，但在数量上不同的突变体，未能完成重组，这表明RPM响应重组状态。与ndj 1所描述的重组缺陷一起，我们的观察表明，RPM和SC分别平衡重组相互作用的破坏和稳定，以调节交叉。
Haploidization of the genome in meiosis requires that chromosomes be sorted exclusively into pairs stabilized by synaptonemal complexes (SCs) and crossovers. This sorting and pairing is accompanied by active chromosome positioning in meiotic prophase in which telomeres cluster near the spindle pole to form the bouquet before dispersing around the nuclear envelope. We now describe telomere-led rapid prophase movements (RPMs) that frequently exceed 1 mu m/s and persist throughout meiotic prophase. Bouquet formation and RPMs depend on NDJ1, MPS3, and a new member of this pathway, CSM4, which encodes a meiosis-specific nuclear envelope protein required specifically for telomere mobility. RPMs initiate independently of recombination but differ quantitatively in mutants that fail to complete recombination, suggesting that RPMs respond to recombination status. Together with recombination defects described for ndj1, our observations suggest that RPMs and SCs balance the disruption and stabilization of recombinational interactions, respectively, to regulate crossing over.